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碳化硅量子点荧光标记技术及其生物学应用
Fluorescence Labeling Technique of Silicon Carbide Quantum Dots and Its Biological Application
【摘要】 通过一步法完成SiC量子点的合成和表面改性,并对其微观结构、光学性质和理化性质进行了表征,结果表明该量子点半径小于激子波尔半径,导致了量子限制效应现象而产生光致发光,通过对其红外光谱的分析发现碳化硅量子点表面既已耦合了巯基,因此该量子点可以作为量子点标记技术中又一种新型的标记材料,然后用SiC量子水相溶液对有、无根皮苷环境下的串珠镰刀菌进行标记并长时程荧光成像,同时让已成功标记的该菌侵染苹果植株幼苗,试验表明,根皮苷能够促进串珠镰刀菌的生长,主要表现在菌落成长的速度和数量上,进一步研究发现,串珠镰刀菌生长态势随周围环境中根皮苷含量的增加而更趋旺盛,此外串珠镰刀菌对苹果幼苗侵染的动态示踪过程表明幼苗的第一感染部位为根毛区。
【Abstract】 The synthesis and surface modification of SiC quantum dots were completed by one-step method. The microstructure,optical properties and physicochemical properties of SiC quantum dots were characterized. The results show that the size of silicon crabide quantum dots was smaller than its exciton Bohr diameter,which leads to the quantum confinement effect,and the photoluminescence is produced.The infrared spectrum shows that the surface of the silicon carbide quantum dots is coupled with the mercapto group,so it can be used as quantum dot labeling technology is another new type of marking materials,then the fluorescence labeling and long-term fluorescence imaging of fusarium moniliforme grown in the presence or absence of phloridzin were carried out by using the silicon carbide quantum dot aqueous solution,and to complete the mark of the bacteria on the apple plant seedlings for infecting,and to fusarium oxysporum which had marked with SiC QDs infecting apple plant seedings,the results show that the technology of labeling of silicon carbide quantum dots achieved the labeling and long-term tracing to fusarium moniliforme successfully,fusarium moniliforme growth slow and slow split in the environment without phloridzine; but grow quickly,split rapidly under the environment with phlorizin,further studies have found that the growth trend of fusarium moniliforme was related to the environment surrounding,the increase in the content of phlorizin. In addition,the dynamic tracing of fusarium moniliforme to apple seedling indicate that the first infection site of the seedlings was root hair.
【Key words】 silicon carbide quantum dots(QDs); fusarium oxysporum; phlorizin; long-term-distance tracing; infecting;
- 【文献出处】 人工晶体学报 ,Journal of Synthetic Crystals , 编辑部邮箱 ,2019年04期
- 【分类号】Q-3;O613.72;TB383.1
- 【被引频次】5
- 【下载频次】182