【摘要】 本实验采用群体感应抑制(quorum sensing inhibitory, QSI)活性导向法,对分离自山东威海近海底泥的真菌WH7-2开展代谢产物研究。综合菌落形态和转录间隔区(Internal Transcribed Spacer, ITS)全序列分析,菌株WH7-2鉴定为腐皮镰刀菌Fusariumsolani。综合运用多种色谱方法,从该真菌大米发酵产物的活性部位中分离得到11个化合物。分别鉴定为(2E, 5E)-3, 5, 7-三甲基-2, 5-辛二烯酸(1)、不饱和脂肪酸酯混合物(2—4)、镰红菌素-3-甲醚(5)、脱水镰红菌素(6)、(22E)-5α, 8α-过氧化麦角甾-6, 22-二烯-3β-醇(7)、(22E)-5α, 8α-过氧化麦角甾-6, 9 (11), 22-三烯-3β-醇(8)、3β-羟基-胆甾-5-烯-7-酮(9)、6β-羟基-胆甾-4-烯-3-酮(10)和(22E)-胆甾-5, 22-二烯-3β-醇(11)。其中不饱和脂肪酸酯混合物(2—4)具有QSI活性,其他化合物无QSI活性。除5和6外,其他化合物均为首次从腐皮镰刀菌F. solani中发现。更多还原

【Abstract】 In this study, we investigated the metabolites from coastal mud-derived fungus WH7-2 by using the quorum sensing inhibitory(QSI) activity-oriented approach. The strain WH7-2 was identified as F. solani by analyzing its morphological characteristics and internal transcribed spacer(ITS) sequence. Chemical investigation of the rice fermentation product led to the isolation of 11 compounds. Their structures were elucidated as(2 E, 5 E)-3, 5, 7-trimethyl-2, 5-octadienoic acid(1), mixtures of unsaturated fatty acid ester(2, 3 and 4), 3-methyl ether fusarubin(5), Anhydrofusarubin(6),(22 E)-5α, 8α-epidioxy-ergosta-6,22-diene-3β-ol(7),(22 E)-5α, 8α-epidioxy-ergosta-6, 9(11), 22-triene-3β-ol(8), 3β-hydroxycholest-5-en-7-one(9),6β-hydroxy-cholest-4-en-3-one(10), and(22 E)-cholest-5, 22-dien-3β-ol(11). All compounds were evaluated for their QSI activities, and only the mixtures of unsaturated fatty acid ester(2-4) exhibited QSI activity. All the compounds except 5 and 6 were first reported from F. solani.更多还原