【摘要】 本文通过纳米ZnO（ZnO-NPs）对具有高效脱氮能力的异养硝化-好氧反硝化菌Halomonas sp. KGL1的短期暴露实验,探讨在不同作用浓度下(0,1,10,50 mg·L^-1) ZnO-NPs对菌株的生物胁迫效应。结果表明,ZnO-NPs破坏菌株Halomonas sp. KGL1的细胞膜完整性并改变其粘滞性,使菌株形态结构改变,菌体发生团聚;同时诱导该菌株细胞产生活性氧（Reactive Oxygen Species,ROS）,对菌株细胞产生氧化胁迫,进一步损伤菌株细胞,抑制菌株的生长和脱氮能力,且ZnO-NPs浓度越高,该菌株受胁迫程度越强。不同浓度的ZnO-NPs对菌株Halomonas sp. KGL1的NH⁺₄-N去除率无显著影响,而其NO^-₃-N、NO^-₂-N的去除效率显著降低。研究结果可为提高海水养殖废水等高盐含氮废水中脱氮菌株的抗ZnO-NPs胁迫能力的理论研究和实际应用提供科学依据。更多还原

【Abstract】 In order to understand the biotic stress of ZnO-NPs on Halomonas sp. KGL1, the authors conducted the short-term exposure experiment of ZnO-NPs with different concentrations(0,1,10,50 mg·L^-1) on heterotrophic nitrification-aerobic denitrifying bacteria Halomonas sp. KGL1 with high denitrification capacity were conducted in this study. The results showed that the integrity of cell membrane is destroyed by ZnO-NPs. And the cells of the strain can be induced to produce ROS, that produces oxidative stress to damage the cells of the strain, and the growth and denitrification ability are inhibited. The higher the concentration of ZnO-NPs is, the stronger the biotic stress of ZnO-NPs on Halomonas sp. KGL1 is. ZnO-NPs of different concentrations have no significant effect on the removal rate of NH⁺₄-N within the range of experimental concentration, but reduced the removal efficiency of NO^-₃-N and NO^-₂-N. The main objective of this study is to provide a scientific basis for the theoretical research and practical application of the ability of denitrifying bacteria to resist ZnO-NPs stress in high-salinity wastewater such as mariculture wastewater.更多还原