【摘要】 本文研究了长期暴露条件下镀镍多壁碳纳米管（MWCNTs-Ni）对序批式反应器（SBR）性能、微生物酶活性和微生物群落的影响。研究结果表明,10 mg/L MWCNTs-Ni的长期暴露未对SBR去除有机物产生影响,而NH₄⁺-N的去除率由（99.10±0.60）%明显降至（39.04±1.61）%。与进水中未加入MWCNTs-Ni时的第32天相比,活性污泥比耗氧速率（SOUR）和脱氢酶（DHA）活性在第148天时分别降低了17.43%和24.32%;而脱氮速率和与脱氮相关的微生物酶活性均降低了60%以上,从而导致SBR对氮的去除效果明显降低。MWCNTs-Ni的长期暴露导致活性污泥活性氧（ROS）产生量和乳酸脱氢酶（LDH）释放量在第148天时分别增加了67.23%和65.33%,表明MWCNTs-Ni的长期暴露能够诱导活性污泥中微生物产生氧化应激和细胞膜损伤。高通量测序结果表明,长期暴露于10 mg/L的MWCNTs-Ni条件下,活性污泥中与硝化过程相关菌属（Nitrosomonas、Nitrosospir、Nitrospira）和与反硝化过程相关菌属（Dokdonella、Dechloromonas、Steroidobacter、Devosia、Thermomonas）的相对丰度明显降低,进而影响到SBR对氮的去除。该研究结果可为评价MWCNTsNi对污水生物处理系统的潜在影响提供一定的理论基础和技术依据。更多还原

【Abstract】 The performance, microbial enzymatic activity and microbial community of a sequencing batch reactor（SBR） were investigated under long-term exposure to Ni coated multi-walled carbon nanotubes（MWCNTs-Ni）. The COD removal had no obvious variation at 10 mg/L MWCNTs-Ni, whereas the NH₄⁺-N removal efficiency decreased from（99.10±0.60）% at 0 mg/L MWCNTs-Ni to（39.04±1.61）% at 10 mg/L MWCNTs-Ni. Compared to 32 d without MWCNTs-Ni, the SOUR and DHA activity decreased by 17.43% and 24.32% at 148 d, respectively, while nitrogen removal rates and microbial enzymatic activity related to nitrogen removal reduced more than 60%, which might lead to the decrease of NH₄⁺-N removal efficiency. The ROS production and LDH release increased by 67.23% and 65.33% at 148 d, respectively, indicating that MWCNTs-Ni could induce oxidative stress and destroy the cytomembrane integrality. High throughput sequencing indicated that the relative abundance of some genera related to nitrification（e.g. Nitrosomonas, Nitrosospir, Nitrospira） and denitrification（e.g. Dokdonella, Dechloromonas, Steroidobacter, Devosia, Thermomonas） decreased at 10 mg/L MWCNTs-Ni, which further effected the nitrogen removal. The present results can provide some theoretical basis and technical support for evaluating the potential impact of MWCNTs-Ni on biological wastewater treatment systems.更多还原