【摘要】 为初步探究反式肉桂醛（TC）对稻绿核菌的抑菌机制,采用透射电子显微镜观察了TC处理后稻绿核菌菌丝细胞的超微结构,并测定了2、4、8、15及30μg/mL系列质量浓度TC对稻绿核菌细胞壁完整性的影响,以及其对烟酰胺腺嘌呤二核苷酸-苹果酸脱氢酶（NADMDHase）、琥珀酸脱氢酶（SDHase）和三磷酸腺苷酶（ATPase）活力的影响。结果显示:经4μg/mL的TC处理后,菌丝细胞内脂质体显著增多,线粒体结构变模糊。当TC质量浓度升高至30μg/mL时,可破坏菌丝细胞壁的完整性,且对NAD-MDHase活力的相对抑制率为44.3%,对SDHase活力的相对抑制率为76.7%;而Na⁺-K⁺-ATPase、Ca²⁺-Mg²⁺-ATPase、Ca²⁺-ATPase及Mg²⁺-ATPase的活力随TC质量浓度升高呈U型变化,其中,4μg/mL的TC对ATPase活力的抑制效果最强,相对抑制率达78.4%以上。研究表明,细胞壁及线粒体可能是TC抑制稻绿核菌菌丝生长的作用靶点,具有进一步研究的价值。更多还原

【Abstract】 The antifungal mechanism of trans-cinnamaldehyde（TC） against Villosiclava virens was investigated. The alteration of cell ultrastructure was observed using transmission electron microscope.The integrity of cell wall and activities of nicotinamide adenine dinucleotide-malic dehydrogenase（NAD-MDHase）, succinate dehydrogenase（SDHase） and adenosine triphosphatase（ATPase） of V.virens were determined after treated with TC at the mass concentration of 2, 4, 8, 15 and 30 μg/mL. The results showed that the contents of lipidosome increased significantly and the structure of mitochondrion was indiscernible after treatment with TC at the mass concentration of 4 μg/mL. With the mass concentration of TC increasing to 30 μg/mL, the integrity of cell wall in V. virens was destroyed and the relative inhibition of NAD-MDHase and SDHase were reduced by 44.3% and 76.7%,respectively. Differently, the activities of Na⁺-K⁺-ATPase, Ca²⁺-Mg²⁺-ATPase, Ca²⁺-ATPase and Mg²⁺-ATPase fluctuated as U-curve with the concentration increase of TC. When the mass concentration of TC was 4 μg/mL, the highest inhibitive activity of TC on ATPase was the achieved among all the tested concentrations and the relative inhibition of TC on ATPase was higher than 78.4%. These results suggested that the cell wall and mitochondrion were the possible action targets for TC to inhibit the mycelial growth.更多还原