【摘要】 为了解牦牛BoLA-I基因mRNA组织表达谱,并探索可能靶定BoLA-I基因的miRNAs,利用RT-PCR技术对牦牛BoLA-I基因mRNA在牦牛肝、脾、肺、肾、肌肉、卵巢、小肠、下颌淋巴结、大肠和肠系膜淋巴结等10种组织的表达谱进行分析,再通过Targetscan和miRBase软件来预测可能靶定BoLA-I基因的miRNAs,检测其在10种牦牛组织中的表达情况。结果表明:BoLA-I基因mRNA在检测的10种牦牛组织中均有表达,尤其在免疫器官组织中广泛表达,在牦牛的肠系膜淋巴结组织表达水平极显著高于小肠和大肠组织(P<0.01);预测到的可能靶定牦牛BoLA-I基因的miRNA共有28个,从中选择了6个进行组织表达谱分析,发现bta-miR-759、bta-miR-142-5p、bta-miR-665、bta-miR-2322-5p、bta-miR-199a-3p与牦牛BoLA-I基因共表达;除卵巢外,bta-miR-219-5p在其他组织中均有表达,且在肝脏组织中的表达水平极显著高于其他组织(P<0.01)。bta-miR-142-5p在肠系膜淋巴结中表达量极显著高于其他组织(P<0.01)。由此推测,牦牛BoLA-I基因及其预测的miRNA可能在牦牛组织中起重要作用。更多还原

【Abstract】 The aims of this study were to understand the mRNA expression profile of yak BoLA-I gene,and to explore possible miRNAs targeting to BoLA-I gene.The mRNA expression of BoLA-I gene in 10 types yak tissue,e.g.liver,spleen,lung,kidney,muscle,ovary,small intestine,jaw,large intestine and mesenteric lymph nodes were investigated by RT-PCR.The possible miRNAs targeting to BoLA-I gene were predicted by TargetScan and miRBase software.Their tissue expression profiles were then analyzed by RT-PCR.The results showed that the mRNA of BoLA-I gene was expressed in all 10 detected yak tissues,especially widely expressed in immune organs.The expression level of mesenteric lymph nodes in yak was significantly higher than that in small intestine and large intestine(P<0.01).It was predicted that there were 28 miRNAs that could target the gene of yak BoLA-I,and 6 miRNAs among them were selected for expression analysis.It was found that bta-miR-759,the bta-miR-142-5 p,bta-miR-665,bta-miR-2322-5 p,and bta-miR-199-a-3 p were co-expressed with yak BoLA-I gene mRNA.bta-miR-219-5 p was expressed in other tissues except for ovaries,and the expression of bta-mir-142-5 p in mesenteric lymph nodes was significantly higher than that in other tissues(P<0.01).The results inferred that the BoLA-I gene and its predicted miRNA might play an important role in yak tissue.更多还原