【摘要】 探讨了Ca²⁺和H₂S调节小麦干旱胁迫的交互关系。结果表明,H₂S和Ca²⁺均可缓解干旱胁迫诱导的氧化伤害,与干旱处理组相比,H₂S和Ca²⁺预处理显著降低了叶片中丙二醛（MDA）和H₂O₂含量,提高了超氧化物歧化酶（SOD）活性,减少了干旱胁迫对过氧化物酶（POD）和过氧化氢酶（CAT）活性的诱导,但乙二醇双（2-氨基乙基醚）四乙酸（EGTA,钙螯合剂）处理显著削弱了H₂S对干旱胁迫的缓解作用,而亚牛磺酸（HT,H₂S清除剂）处理对干旱胁迫下Ca²⁺的缓解作用没有影响。此外,干旱胁迫下,H₂S可诱导Ca²⁺信号途径相关基因表达（如CcaMK,CPK10,CIPK2和CIPK15）,而Ca²⁺对小麦叶片内源H₂S含量无显著影响。由此推测,Ca²⁺可能位于H₂S信号的下游,部分介导了H₂S对小麦干旱胁迫的调节作用,H₂S可通过促进Ca²⁺信号途径相关基因的表达调节干旱胁迫。更多还原

【Abstract】 The interaction between calciumion(Ca²⁺) and hydrogen sulfide（H₂S） in regulation drought stress for wheat was investigated in this paper. The results showed that both H₂S and Ca²⁺ could relieve the drought-induced oxidative damage. Compared with the drought-treated group, H₂S and Ca²⁺ pretreatment significantly reduced the content of MDA and H₂O₂ in the leaves, increased SOD enzyme activity and decreased drought-inducted POD and CAT activities. However, the treatment of EGTA（calcium chelator） significantly attenuated the alleviating effect of H₂S on drought stress, while, HT（H₂S scavenger） treatment did not affect the regulation of Ca²⁺ on drought stress. In addition, under drought stress, H₂S could induce the expression of Ca²⁺ signaling pathway-related genes（such as CcaMK, CPK10, CIPK2 and CIPK15） under drought stress, but Ca²⁺ had no significant effect on the production of endogenous H₂S in wheat leaves. These results showed that Ca²⁺ may be located downstream of the H₂S signal, partially mediates drought stress regulation by H₂S, H₂S may regulate drought stress by promoting the expression of Ca²⁺ signaling related genes.更多还原