【摘要】 为研究阿尔山地区落叶松根际固氮菌的多样性,试验采用平板分离法,结合16S r DNA序列比对,对落叶松根际土壤固氮菌组成类群及其多样性进行了分析。试验结果表明:自落叶松根际土壤共分离纯化细菌菌株28株,分属于10属18种,分别为假单胞菌属(Pseudomonas)、沙雷菌属(Serratia)、伯克氏菌属(Burkholderia)、根瘤菌属(Rhizobium)、叶杆菌属(Phyllobacterium)、类芽孢杆菌属(Paenibacillus)、芽孢杆菌属(Bacillus)、红球菌属(Rhodococcus)、小杆菌属(Curtobacterium)、节杆菌属(Arthrobacter)。其中Pseudomonas、Phyllobacterium、Arthrobacter为优势菌属。研究结果阐明了阿尔山地区落叶松根际固氮菌的组成及其多样性。更多还原

【Abstract】 In this paper plate isolation method,combined with 16 S r DNA sequence analysis,were used to investigate the diversity and community structure of rhizosphere nitrogen-fixing bacteria isolated from Larix gmelinii in Arxan National Forest Park. A total of 28 isolates were obtained from L. gmelinii rhizosphere soil and their 16 s rDNAs were sequenced and analyzed. The isolates were classified into 10 genera and 18 species. The genera isolated included Pseudomonas,Serratia,Burkholderia,Rhizobium,Phyllobacterium,Paenibacillus,Bacillus,Rhodococcus,Curtobacterium and Arthrobacter,with Pseudomonas,Phyllobacterium,Arthrobacter are dominant groups.The results showed the community structure and diversity of nitrogen-fixing bacteria in the rhizosphere soil of L. gmelinii in Arxan National Forest Park.更多还原