【摘要】 microRNAs可作为肿瘤抑制因子或促进因子发挥作用,其表达与肿瘤的发生发展密切相关.miR-2基因家族成簇表达,是果蝇基因组中编码microRNA的最大的基因家族,但其功能不确定.为了研究miR-2基因家族的功能,本研究利用CRISPR/Cas9系统,成功敲除了miR-2家族位于2号染色体左臂(2L)的基因簇(ΔmiR-2),并初步确定了ΔmiR-2可以抑制促癌因子Yki活性,为研究miR-2家族的功能奠定了基础.更多还原

【Abstract】 MicroRNAs function as tumor suppressors or promoters during tumor progression. The largest Drosophila microRNA gene family miR-2 consists of 8 members and 6 of them form two gene clusters on different chromosomes. However, the function of this gene family is unknown. In this study, we generated a specific genome deletion of miR-2 cluster located on chromosome 2 L using the CRISPR/Cas9 system in Drosophila. This knockout strain(ΔmiR-2) genetically suppress the activity of oncogenic factor Yki, indicating ΔmiR-2 will facilitate the functional study of miR-2 in the future.更多还原