【摘要】 腺毛作为植物表皮细胞的特化结构,在植物与环境互作中起重要作用。为探究不同类型腺毛在镉(Cd)胁迫中所发挥的作用,以非分泌型烟草品种T.I.1112和分泌型烟草品种T.I.1068为材料,研究二者对Cd胁迫的耐受性差异、腺毛形态和叶片中Cd积累的动态变化规律。结果发现,分泌型烟草品种T.I.1068对Cd的耐受性明显高于非分泌型烟草品种T.I.1112。显微观察发现,Cd胁迫下分泌型烟草品种和非分泌型烟草品种叶面腺毛密度都明显增加,但腺毛类型保持不变。电感耦合等离子体发射光谱(ICP-OES)检测发现,烟叶中的总Cd含量随处理时间延长而逐渐增加,分泌型烟草品种和非分泌型烟草品种叶片的总Cd含量基本一致,但二者叶表面的Cd含量存在显著差异,分泌型烟草叶面的Cd含量远远大于非分泌型烟草。表明分泌型腺毛能排出烟叶中的Cd,降低烟叶中的镉含量,对提高烟草对Cd胁迫的耐受性具有重要作用,结果为烟草品种改良、提高烟草制品安全性及土壤修复等方面提供理论基础。更多还原

【Abstract】 As a specialized structure of plant epidermal cells,trichomes play an important role in plant-environment interaction. In present work,non-glandular tobacco variety T.I.1112 and glandular tobacco variety T.I.1068 were used to study the difference between glandular and non-glandular trichomes in response to cadmium( Cd) stress. By comparing the differential performance between T.I.1112 and T. I.1068 under Cd stress,it was found that T. I.1068 enhanced tolerance to Cd stress. The results of inductively coupled plasma optical emission spectrometry( ICP-OES)showed that the total Cd content in tobacco leaves gradually increased along with Cd treatment time,with the final Cd content in T.I.1112 and T.I.1068 showed no obvious difference,but the secreted Cd on leaf surface differed greatly,with much higher Cd content on leaf surface of glandular tobacco than non-glandular tobacco. These results indicated that glandular trichomes play more important role in improving tobacco tolerance to Cd by excluding toxic Cd outside the plant. Above results provided references for further work on tobacco breeding,cigarette safety improvement and soil remediation.更多还原