【摘要】 文章以小新月菱形藻（Nitzschia closterium f. minutissima）为研究对象,分析比较了小新月菱形藻在负压光生物反应器与开放式桶培养下,藻密度、pH、溶解氧及菌落结构的变化情况。结果表明,在负压光生物反应器培养下的藻密度可达到1.33×10⁷个·mL^–1,明显高于开放式培养的藻密度(8.36×10⁶个·mL^–1)。藻液中pH随藻密度增加而升高,两者呈显著正相关（P<0.01）,在负压光生物反应器及开放式培养环境中pH最高值分别为10.3和9.3。溶解氧与pH变化趋势相反,在负压光生物反应器内溶解氧随藻密度增加而降低,最后稳定在6.5 mg·L^–1,溶解氧的下降可能与玫瑰杆菌（Roseobacter）成为优势细菌有关。利用16S rDNA基因的高通量测序技术,分析在培养过程中藻际菌群的结构变化,发现菌落的多样性显著下降（P<0.05）,培养前期主要以变形杆菌（Proteobacteria）和拟杆菌（Bacteroidetes）为优势细菌,在负压光生物反应器内培养后期主要以蓝细菌（Cyanobacteria）与玫瑰杆菌为优势细菌,其菌落结构与开放式桶存在明显差异。更多还原

【Abstract】 We compared the density, pH, dissolved oxygen and colony structure of Nitzschia closterium f. minutissima bred in negative pressure photobioreactor and open bucket, respectively. It is shown that the algae grew fast in negative pressure photobioreactor with the highest density of 1.33×10⁷ cells·mL^–1, significantly higher than that in open bucket(8.36×10⁶ cells·mL^–1). The pH in algal solution increased with increase of algal density, and there was a significant positive correlation between them（P<0.01）. The highest pH values in negative pressure photobioreactor and open bucket were 10.3 and 9.3, respectively. Oppositely, in negative pressure photobioreactor, the dissolve oxygen of algea decreased with increase of algal density, which finally stabilized at about 6.5 mg·L^–1. The decline of dissolved oxygen might be related to the fact that Roseobacter had become the dominant bacteria. Results of 16S rDNA gene high-throughput sequencing show that the bacterial diversity decreased significantly（P<0.05）. Proteobacteria and Bacteroidetes were dominant bacteria at early stage of breeding. However, the dominant bacteria were Cyanobacteria and Roseobacter at late stage in negative pressure photobioreactor, which was obviously different from that in open bucket.更多还原