【摘要】 采用螺旋藻(Spirulina platensis)添加量分别为0 (对照组)、1%(T1)、2%(T2)、3%(T3)、4%(T4)和5%(T5)的6组实验饲料,养殖均质量为(25.49±0.20) g的花鲈(Lateolabrax maculatus) 8周,研究其对花鲈生长、消化酶、血液学指标及抗氧化能力的影响。结果显示,添加螺旋藻可显著提高花鲈特定生长率和增重率,并显著降低饲料系数(P<0.05);螺旋藻可以提高花鲈肠道蛋白酶活性(P<0.05),但对淀粉酶和脂肪酶活性均无显著影响;T3、T4和T5组的白细胞数和血红蛋白浓度、T4和T5组的红细胞数均显著高于对照组(P<0.05);T4和T5组的总胆固醇、甘油三酯和低密度脂蛋白胆固醇浓度显著低于对照组(P<0.05);T4和T5组的溶菌酶活性、免疫球蛋白M和补体4浓度显著高于其他组(P<0.05),T3、T4和T5组的总抗氧化能力、过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶活性显著高于对照组(P<0.05),T3、T4和T5组的丙二醛浓度显著低于对照组(P<0.05)。综上,花鲈饲料中螺旋藻的适宜添加量为4%～5%。更多还原

【Abstract】 Chinese sea bass(Lateolabrax maculatus) with initial body mass of(25.49±0.20) g were fed for eight weeks with six diets containing 0(control), 1%(T1), 2%(T2), 3%(T3), 4%(T4) and 5%(T5) Spirulina platensis, so as to investigate the effect of S. platensis on the growth performance, digestive enzymes, haematological indices and antioxidant capacity of L. maculatus. The results show that the dietary supplementation with S. platensis improved the weight gain rate(WGR) and specific growth rate(SGR) of L. maculates(P<0.05) significantly. The feed conversion ratio(FCR) decreased significantly(P<0.05). The protease activities in S. platensis supplemented groups were significantly higher than that of the control(P<0.05), while no significant change was observed in lipase and amylase activities among different groups. Compared with the control, the levels of red blood cell(RBC) increased in T4 and T5 groups significantly(P<0.05), and levels of white blood cell(WBC) and hemoglobin(Hb) in T3, T4 and T5 groups were significantly higher than those of the control(P<0.05). The levels of triglyceride, cholesterol, low-density lipoprotein cholesterol(LDL-C) in T4 and T5 groups decreased significantly than those of the control(P<0.05). The total antioxidant capacity(T-AOC), superoxide dismutase(SOD), catalase(CAT) and glutathione peroxidase(GSH-Px) activities in T3, T4 and T5 groups were significantly higher than those of the control(P<0.05). The malondialdehyde(MDA) contents in T3, T4 and T5 groups were significantly lower than those of the control(P<0.05). It is suggested that the suitable S. platensis supplementation level in diet of L. maculates is 4%-5%.更多还原