【摘要】 地下水细菌群落结构和多样性是反映其生态系统环境敏感性的重要指标。为研究工业园区地下水细菌群落结构及多样性,于2016年12月对常州市某工业园区地下水进行采集,采用高通量技术对细菌的16S rRNA基因进行序列测定。高通量测序结果表明工业园区地下水细菌群落呈现出典型的淡水种群特征,主要由变形菌门(Proteobacteria,86. 40%)、拟杆菌门(Bacteroidetes,5. 06%)和厚壁菌门(Firmicutes,3. 04%)等55个细菌门,γ-变形菌纲(Gammaproteobacteria,47. 51%)、β-变形菌纲(Betaproteobacteria,29. 58%)和α-变形菌纲(Alphaproteobacteria,6. 75%)等95个细菌纲以及假单胞菌属(Pseudomonas,16. 38%),Perlucidibaca (9. 17%)和不动杆菌属(Acinetobacter,8. 12%)等564个细菌属组成。经分析,水质情况影响着细菌群落组成和多样性,水质较好的点位细菌群落丰富度和均匀度也较高;冗余分析结果表明氯化物、钙、电导率、铁、氟化物和硫酸盐是能显著解释地下水细菌群落的环境因子。更多还原

【Abstract】 The structure and diversity of bacterial community in groundwater are important indicators to reflect the environmental sensitivity of the ecosystem. In order to investigate the bacterial community structure and diversity of the groundwater in an industrial park,the groundwater samples from an industrial park in Changzhou were collected in December2016,and the 16 S rRNA gene of bacteria was sequenced by high-throughput sequencing. The results show that bacterial community structure of the groundwater present a typical characteristics of freshwater populations and could be divided into55 phyla,95 classes and 564 genera. Proteobacteria( 86. 40%),Bacteroidetes( 5. 06%) and Firmicutes( 3. 04%) are the dominant phyla in the studied groundwater. The abundant classes within the phyla Proteobacteria are Gammaproteobacteria( 47. 51%),Betaproteobacteria( 29. 58%) and Alphaproteobacteria( 6. 75%),respectively. At the genus level,Pseudomonas( 16. 38%),Perlucidibaca( 9. 17%) and Acinetobacter( 8. 12%) are predominant. The influence of the physical and chemical indicators on bacterial community show that the water quality affects the composition and diversity of bacterial community,and the better the water quality is,the higher the richness and evenness of bacterial community are.Redundancy analysis results show that chloride,calcium,conductivity,iron,fluoride and sulfate are the environmental factors that can significantly explain the groundwater bacterial community.更多还原