【摘要】 为表征绿潮藻浒苔腐烂过程中产生的次生毒性危害,采用一种基于费氏弧菌(Vibrio fischeri)的应急毒性检测系统,在81. 9%低毒性测试模式下,对浒苔腐烂液进行次生毒性检测。在实验室条件下,往自然海水中按等比倍数添加不同生物量的浒苔(2 g/L、10 g/L、50 g/L)模拟其腐烂分解过程,定期检测腐烂液次生毒性。研究发现:使用费氏弧菌作为发光细菌在绿潮次生毒性检测中,可以较好地反映绿潮次生毒性的变化过程;一定体积的海水中,浒苔腐烂液毒性的变化与浒苔生物量密切相关;添加的浒苔量越高,重毒和剧毒出现的时间越早,持续的时间越长,毒性消退的过程越缓慢。毒性等级天数百分比结果显示,添加浒苔生物量越高的试验组剧毒和重毒等级天数占比越大; A组、B组、C组、D组合计出现剧毒和重毒等级天数比例分别为0%、18%、60%、100%。高浓度浒苔腐烂会产生明显的次生毒性,并且其毒性消退过程缓慢,应在浒苔绿潮发生海域及时清理浒苔,加强水质监测,防范浒苔腐烂分解造成养殖危害。更多还原

【Abstract】 In order to characterize the secondary toxic hazard of Ulva prolifera Green tide in decomposition process,an emergency toxicity detection system based on Vibrio fischeri was used in 81. 9% low toxicity test mode.2 g/L,10 g/L and 50 g/L of Green alga U. prolifera by equal ratio were added to natural sea water to simulate the decomposition process in laboratory. The secondary toxicity was detected regularly. The study shows that Vibrio fischer as a luminescent bacteria can accurately reflect the level and the chang of secondary toxicity in U. prolifera Green tide decomposition. In a certain volume of seawater,the change of toxicity is closely relation to the biomass of U. prolifera. Compared with the low biomass of U. prolifera,the emergence of highly posion and rank posion are earlier,the duration of toxicity is longer and the process of toxicity regression is slower for the high biomass of U.prolifera. The result shows that the percentage of rank posion and highly posion grade are accounting for a larger rate in higher biomass of Green alga U. prolifera. The total proportions of rank posion and highly posion days in Group A,B,C,D are 0% 、18% 、60% 、100% respectively. The higher biomass of U. prolifera decomposition produces obvious secondary toxicity and its toxicity degradation process is slower. It’s necessary to clean up the Green tide timely and strengthen water quality monitoring to prevent the harmness of decomposition of Green tide to aquaculture.更多还原