【摘要】 为研究褐飞虱响应麦黄酮刺激的分子机理,应用差异蛋白质组学方法分析褐飞虱唾液腺响应麦黄酮刺激处理的蛋白质组变化趋势。结果表明,麦黄酮处理褐飞虱6 h差异蛋白数量90个,其中上调蛋白62个,下调蛋白28个;处理褐飞虱24 h差异蛋白数量27个,其中上调蛋白10个,下调蛋白17个,6 h的差异蛋白数量显著大于24 h。麦黄酮处理可以引起褐飞虱唾液腺丝氨酸蛋白酶及其同系物产生显著变化。通过对差异蛋白进行GO功能分析和KEGG通路分析,发现麦黄酮处理褐飞虱引起褐飞虱唾液腺蛋白变化主要集中在应激蛋白和代谢这两大类上,分别占了所有差异蛋白的50%以上,麦黄酮处理引起褐飞虱唾液响应蛋白主要参与了氨基酸代谢、唾液分泌系统、能量代谢、碳水化合物代谢等通路。更多还原

【Abstract】 In order to research the molecular mechanism of rice brown planthopper Nilaparvata lugens response to tricin stimulation, we analyzed the salivary proteomic response of N.lugens to tricin stimulation by using differential proteomic methods. The results showed that the differential proteins in N.lugens salivary treated with tricin for 6 hours reaches 90(including 62 up-regulated proteins and 28 down-regulated proteins). The differential proteins inside the N.lugens sample treated with tricin for 24 hours significantly less than treated for 6 hours, and the number of different protein just was 27(including 10 up-regulated proteins and 17 down-regulated proteins). Tricin stimulation can make serine protease(SP) and its homologous(SPH) inside the rice brown planthopper salivary gland changing obviously. Through by GO function analysis and KEGG pathway analysis towards the differential proteins, we found that the differential proteins in N.lugens salivary gland mainly belonged to stress proteins and metabolism proteins, and total of their ratio more than 50 percent. The N.lugens salivary gland proteins responsed to tricin stimulation participating the following pathways, such as amino metabolism, salivary secretion system, energy metabolism, carbohydrate metabolism, and so on.更多还原