【摘要】 尖孢镰刀菌古巴专化型Fusarium oxysporum f. sp. cubense(FOC)是威胁香蕉生产的重要土传病原真菌,其厚垣孢子可在土壤中存活多年,是香蕉枯萎病的重要侵染源。为解析该病菌厚垣孢子的形成机制,本研究建立了厚垣孢子的诱导形成体系。通过氨基糖添加试验,证明添加N-乙酰葡糖胺可抑制厚垣孢子的形成;通过对该病菌厚垣孢子形成前期(0h)、初期(24h)、中期(48h)和后期(96h)的转录组分析,发现氨基糖代谢通路中有41个基因的表达水平在厚垣孢子形成过程中发生了变化,其中9个基因与几丁质合成相关,32个基因与糖类化合物的合成和代谢及催化转换相关。本研究首次证明了氨基糖代谢通路与尖孢镰刀菌古巴专化型厚垣孢子形成的相关性。更多还原

【Abstract】 Fusarium oxysporum f. sp. cubense(FOC) is an important soil-borne pathogenic fungus, which leads to a serious threat to the production of banana in the world. Vast amount of chlamydospores produced by the pathogen are able to survive many years in the infected-soil, which become major infectious sources of this disease. To explore the mechanisms of chlamydospore formation in FOC, an induction system was set up. The result was that the chlamydospore formation of FOC was inhibited by the supplement of N-acetylglucosamine in the induction system. Transcriptome analyses of four chlamydospore formation stages(mycelial stage, 0 h;initial stage, 24 h; middle stage, 48 h; later stage, 96 h) revealed that there were 41 genes varying their expression levels in amino sugar metabolism pathway. Among which, nine genes were related to chitin synthesis and 32 genes were related to synthesis, metabolism and catalytic conversion of carbohydrates. This study confirmed that amino sugar metabolism pathway was involved in chlamydospore formation of FOC.更多还原