【摘要】 为明确辣椒根腐病病原,从田间采集分离、纯化、鉴定、柯赫氏法则验证病原菌,并开展高氏15号生物菌剂对辣椒根腐病的防治效果和促生作用研究.结果表明,通过形态学和分子生物学鉴定(ITS、EF-1a、IGS基因序列比对及进化树分析)发现,引起该地区辣椒根腐病的病原物为茄病镰刀菌(Fusarium solani).柯赫氏法则完成了辣椒根腐病的回接鉴定试验.田间防效和促生结果显示,多粘类芽孢杆菌在240g/667m2,高氏15号菌剂在50g/穴和75g/穴,甲霜·恶霉灵1 500倍液防治辣椒根腐病效果最好,均达到70%以上.经试验处理的株高、根长、整株鲜重、根鲜重和根干重和空白对照相比有显著差异性.更多还原

【Abstract】 To identify the pathogen of pepper root rot,the pathogenic fungi was isolated and purified from pepper stalk root disease.The pathogenic fungus was verified via molecular determination and Koch’s Rule.Field control and growth promotion effect were achieved to prevent pepper root rot by Gauze No.15.The result showed that the pathogen causing root rot of pepper was identified as Fusarium solani through identification of morphological characteristics and molecular biological analysis including ITS,EF-1 aand IGS genes and phylogenetic tree in this area.Koch’s Rule was finished by infestation test.In addition,the result indicated that applying 240 g/667 m2 of Paenibacillus polymyxa,50 g/den and 75 g/den of Gause No.15 and 3% hymexazol metalaxyl AS(1 500×)generated the optimal inhibition effect on the Fusarium solani(more than 70%).After treatment,the pepper height,root length,fresh weight of the whole plant and root,and dry weight of the root had significant difference comparing with control group.更多还原