【摘要】 将抗高致病性副溶血弧菌(Vibrioparahaemolyticus)特异性卵黄抗体(AHPND-VpIgY)应用于育苗生产,通过测定氨氮、亚硝酸盐、细菌总数、弧菌总数、相对保护率和育苗成活率,评价了AHPND-VpIgY在育苗期对凡纳滨对虾(Litopenaeusvannamei)幼体的保护效果。结果表明,0.1g/m3和0.2g/m3AHPND-VpIgY组的相对保护率为35.7%和57.2%,两者均显著高于非特异性卵黄抗体组(P<0.05);两组的育苗成活率为50.8%和57.2%,比对照组分别提高19.1%和25.6%。各实验组氨氮、亚硝酸盐、细菌总数及弧菌总数与对照组无显著差异,对水质无影响。综上,特异性的AHPND-VpIgY能在不改变水质的前提下,有效提高凡纳滨对虾幼体的存活率、相对保护率和育苗成活率,从而提高凡纳滨对虾苗种产量和质量。更多还原

【Abstract】 We applied the specific egg yolk antibody of highly pathogenic Vibrio parahaemolyticus(AHPND-VpIgY)for seedling breeding of Litopenaeus vannamei. The protective effect with the antibody was evaluated by measuring water quality, total bacteria, total Vibrio count, relative protection rate, and seedling survival rate. The results show that the relative protection rate of the 0.1 g/m3 and 0.2 g/m3 AHPND-VpIgY groups was 35.7% and 57.2%, respectively, which is higher than that of the control(no AHPND-VpIgY)(P<0.05). The larva survival rate of the 0.1 g/m3 and 0.2 g/m3 AHPND-VpIgY groups was 50.8% and 57.2%, which was 19.1% and 25.6% higher than that of the control group,respectively. Meanwhile, no significant difference in ammonia nitrogen, nitrite, total bacteria, and total Vibrio count were found between the experiment groups and control group. Therefore, application of AHPND-VpIgY could increase the survival rate of the larva, the relative protection rate, and the survival rate of seedling, and enhance the yield and quality of L. vannamei seedlings while the water quality remained unchanged.更多还原