【摘要】 在不同氮浓度条件下对红色赤潮藻(Akashiwosanguinea)进行了一次性培养,研究了其生长过程中脂肪酸含量及组成变化。结果表明:红色赤潮藻脂肪酸含量及组成受初始氮浓度及生长阶段的影响。低氮处理组(0—25μmol/L)脂肪酸含量[(150.02±54.84)—(166.83±46.46)mg/gdw]显著高于其他处理组(P<0.05),883μmol/L组最低,约为(86.70±5.66)mg/gdw;各培养组脂肪酸含量随着培养时间的延长而增加,稳定期时脂肪酸含量最高,指数生长前期含量最低。红色赤潮藻含28种脂肪酸,以C16:0、C18:1、C20:5ω3(EPA)和C22:6ω3(DHA)为主,且DHA相对含量较高,最高可占总脂肪酸含量的30.46%。随着初始氮浓度的增加,红色赤潮藻饱和脂肪酸(SFA)和单不饱和脂肪酸(MUFA)在总脂肪酸中所占比例下降,而多不饱和脂肪酸(PUFA)所占比例升高。在红色赤潮藻生长过程中,MUFA相对含量在稳定期时较高,而PUFA相对含量在指数生长前期较高。研究结果可为进一步揭示红色赤潮藻赤潮应对环境胁迫的生理响应机制提供依据。更多还原

【Abstract】 Batch cultures of the planktonic dinoflagellate Akashiwo sanguinea were conducted in different nitrate concentrations. Variations of the fatty acid content and its composition at different growth stages of A. sanguinea were analyzed. The results indicate that the fatty acid content and composition varied with both N concentration and growth stage. In particular, the fatty acid contents in lower nitrogen concentrations [0—25μmol/L,(150.02±54.84)—(166.83±46.46)mg/g dw] were significantly higher than those in other N treatments(P<0.05). The lowest value(86.70±5.66)mg/g dw was found in the 883μmol/L treatment. The fatty acid content in each treatment increased with the growth of A. sanguinea, reaching the highest in the stationary growth phase and lowest in the early exponential growth phase. Twenty-eight 28 fatty acids were detected in A. sanguinea, including mainly C16:0, C18:1, C20:5ω3(EPA), and C22:6ω3(DHA). The DHA content was relatively higher, with the maximum of 30.46% of total fatty acids. With the increases of initial N addition, the proportions of saturated fatty acid(SFA) and monounsaturated fatty acid(MUFA)declined whereas the polyunsaturated fatty acid(PUFA) increased. In addition, the proportion of MUFA was higher in the stationary phase, while PUFA was higher in the early exponential growth phase. The results help further understanding of the physiological strategy of A. sanguinea adoption to an environmental stress.更多还原