【摘要】 为寻求更高效、实用的生物脱毒制剂,提高其应用范围,通过采集酸性、高温的温泉环境样品,筛选获得1株高效降解玉米赤霉烯酮（ZEN）的细菌菌株Y-33,并对该菌株生长和降解特性进行分析。结果表明,菌株Y-33在50℃、pH值5.0条件下孵育36 h,菌液对2μg·mL^-1 ZEN的降解率高达93.79%,对20μg·mL^-1 ZEN的降解率也达82.40%。从形态、生理生化特性及16S rDNA序列对菌株Y-33进行分析,初步鉴定为枯草芽孢杆菌（Bacillus subtilis）。该菌株发酵上清液在28℃条件下对ZEN降解率为62.02%。金属离子Mn²⁺能明显增强菌株Y-33发酵上清液对ZEN的降解能力,降解率达81.17%,而Zn²⁺严重抑制了Y-33发酵上清液对ZEN的降解能力,降解率仅为5.42%。本研究结果为ZEN解毒菌剂的开发与应用奠定了理论基础。更多还原

【Abstract】 To seek more efficient and practical biological detoxification strain to improve the application scope, we collected samples from acid and high temperature environment. One strain of bacteria named Y-33, was isolated for efficient detoxification zearalenone（ZEN）,. Y-33 strain was incubated at 50℃ and pH value 5.0 for 36 hours, after which the degradation rate of bacterial solution to 2 μg·mL^-1 ZEN was as high as 93.79%, and up to 82.40% to 20 μg·mL^-1 ZEN. The strain Y-33 was initially identified as Bacillus subtilis with the analysis of its morphological, physiological and biochemical characteristics and 16 S rDNA sequences. The degradation rate in fermentation supernatant of the strain′s was 62.02% at 28℃. Metal ion Mn²⁺ could significanty enhance the degradation ability of strain Y-33 culture supernatant to ZEN with the degradation rate being 81.17%, while Zn²⁺ severely inhibited the degradation capability of ZEN by Y-33 culture supernatant to only 5.42%. The results of this study lay the theoretical foundation for the development and application of ZEN detoxifying microbes.更多还原