【摘要】 为探究苯并芘（BaP）对鲤鱼的毒性机制,本研究检测了不同浓度BaP(0、0.1、0.5、1.0μg·L^-1)暴露对鲤鱼肝、肾组织中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽（GSH）、丙二醛（MDA）及非特异性免疫酶溶菌酶（LZM）、酚氧化酶（PO）、酸性磷酸酶（ACP）、碱性磷酸酶（AKP）活性,以及硝基四氮唑兰（NBT）阳性细胞及肝、肾组织结构的影响。结果表明,1.0μg·L^-1BaP暴露30 d后,鲤鱼肝、肾组织SOD活性、MDA含量及肝组织CAT、肾组织GSH活性均显著低于对照组;1.0μg·L^-1Ba P暴露的肝、肾组织LZM、AKP和PO活性以及肝组织ACP活性均显著低于对照组;不同浓度Ba P暴露30 d后,鲤鱼NBT阳性细胞数均显著下降,且对肝、肾组织造成不同程度的损伤。相关性分析结果表明,Ba P暴露浓度与肝、肾组织的SOD活性呈负相关,与MDA含量呈正相关,与肝、肾组织的AKP、ACP、PO活性呈负相关,与NBT阳性细胞数呈负相关。综上,水体中Ba P暴露对鲤鱼肝、肾抗氧化和非特异性免疫能力及组织结构产生了一定程度的负面影响,本研究为水体中Ba P的生态毒性效应研究提供了理论参考。更多还原

【Abstract】 To investigate the toxic mechanism of BaP on carp（Cyprinus carpio）,the antioxidases（SOD,CAT,GSH）activities,MDA content,and the activities of non-specific immunity enzymes（LZM,PO,AKP,ACP）,NBT positive cells and organization structure in liver and kidney of carp exposed to different concentrations of BaP(0,0.1,0.5,1.0μg·L^-1)for 30 days were studied.The results showed that after exposure for 30 days,SOD activity and MDA content in liver and kidney,CAT activity in liver,and GSH activity in kidney exposed to 1.0μg·L^-1of BaP were significantly lower than those in the control group.The activities of LZM,AKP and PO in liver and kidney,and ACP activity in liver exposed to 1.0μg·L^-1of BaP were significantly decreased compared with control group.The number of NBT positive cells decreased significantly compared with control group.The liver and kidney tissue were damaged to different degrees.Correlation analysis indicated that the BaP concentration was negatively correlated with SOD activities,was positively correlated with MDA content in liver and kidney,had a negative correlation with the activities of AKP,ACP,PO in liver and kidney,was negatively correlated with the number of NBT positive cells.All above indicated that exposure to BaP in water could negatively affect the antioxidant ability,non-specific immunity function and organization structure in liver and kidney of carp to some extent,which provided the important information for further study of the ecological toxicity of BaP.更多还原