【摘要】 将天麻水提取液经过NKA–9大孔吸附树脂与制备型液相色谱联用方法分离,获得6个主要活性成分,通过质谱仪和核磁共振仪鉴定其结构分别为天麻素、对羟基苯甲醇、巴利森苷E、巴利森苷B、巴利森苷C、巴利森苷A;采用HPLC方法测定不同产地初加工样品中这些活性成分的含量,杀青后干燥与不杀青直接干燥的天麻活性成分含量间的差异有统计学意义,不杀青样中对羟基苯甲醇含量显著高于杀青样中的含量,其他活性成分含量则显著低于杀青样中的含量;蒸、煮2种杀青方法中6个活性成分及总含量间的差异均无统计学意义,煮法、蒸法、不杀青处理的天麻活性成分总含量依次降低;切片与整块干燥处理样中,各活性成分及总含量间的差异均无统计学意义,但切片干燥的天麻活性成分总含量高于整块干燥的含量;不同干燥温度处理样中,各活性成分及总含量间的差异均无统计学意义,70、60、50℃温度干燥的天麻活性成分总含量依次降低。新鲜天麻煮法杀青,切片,在70℃干燥下活性成分总含量最高,为较好的初加工方法。更多还原

【Abstract】 Six active components,gastrodin,hydroxybenzyl alcohol,parishin E,parishin B,parishin C and parishin A,have been collected by use of the combination NKA-9 macroporous resin with preparative liquid chromatography from the water extract of Gastrodia elata Blume.With HPLC,there were no significant differences in steaming and boiling samples on the six active components and total amounts,but the both processing method were significant different from the method of non fixing sample for the six components.The compound of hydroxy benzyl alcohol was significantly lower in steaming and boiling samples,the other components were reverse;there was no significant difference in the 6 active components and total amounts between the two methods of steaming and boiling,the total amounts of active components treated by boiling,steaming and not fixing was successively reduced;there was no statistically significant difference between the active components and the total amounts of the drying with slice and drying with whole,but the total amounts of the active components in the drying with slice was higher than that in the drying with whole;there is no significant difference between the different drying temperatures,the total amounts of active components dried by 70,60 and 50 ℃ was successively reduced.Fresh Gastrodia elata Blume has the highest content of active components in boiling,slicing and drying at 70 ℃,which is the better primary processing method.更多还原