【摘要】 采用移动床生物膜反应器（MBBR）-膜生物反应器（MBR）组合工艺进行生活污水处理试验,考察了组合工艺对污染物的去除效果,通过变性梯度凝胶电泳（DGGE）分析反应器内微生物种群组成及多样性变化,利用克隆文库分析微生物种群结构。结果表明:MBBR-MBR组合工艺稳定运行时对氨氮（NH4+-N）和化学需氧量(COD_Cr)的平均去除率高达98%和97%;DGGE指纹图谱显示,反应器运行过程中微生物群落结构演变明显且与温度变化密切相关,逐渐形成适应污水处理的低温稳定种群,MBBR与MBR相比,其反应器内微生物种群更丰富,抗冲击负荷能力更强;克隆文库结果显示,MBBR与MBR内微生物优势种有较大差异,但同属变形菌门（Proteobacteria）。更多还原

【Abstract】 The moving bed biofilm reactor（ MBBR）-membrane bioreactor（ MBR） combined process was used for the treatment of domestic wastewater. The removal effect of the reactors on the pollutants was investigated, the microbial population changes and compositional diversity in the reactors were analyzed by denaturing gradient gel electrophoresis（ DGGE）, and the microorganisms were classified by using cloned library. The results showed that the NH4+-N and COD_Crin MBBR-MBR could acquire satisfied removal efficiency under stable operation, which were 98% and 97%. DGGE fingerprint showed that during the operation of the reactor, the microorganism community structure evolved obviously and was closely related to the temperature change, gradually forming a low temperature stable population adapted to the sewage treatment. Compared with MBR, MBBR had richer microbial population, more complex system and stronger resistance. The results of cloned library showed that the dominant species of microorganisms in MBBR and MBR were quite different, but both belonged to Proteobacteria.更多还原