【摘要】 目的探讨夏季与冬季的大气颗粒物PM_2.5水溶性成分与总颗粒物对BEAS-2B细胞的细胞活性与炎症的影响。方法将夏季和冬季采集的PM_2.5分别提取水溶性成分与总颗粒物,得到4种PM_2.5组分,使用CCK-8法检测25、50、100、200、400μg/ml PM_2.5暴露细胞后的细胞活性;以100μg/ml PM_2.5处理细胞12、24、48 h后提取RNA,用qRT-PCR技术检测IL-1α、IL-1β、IL-6、IL-8炎症因子与炎症小体NLRP3及caspase-1、IL-18的水平。结果夏季和冬季PM_2.5的水溶性成分与总颗粒物分别处理细胞12 h后,部分剂量组细胞活性升高,随着PM_2.5暴露时间的增加,细胞活性逐渐得到抑制,差异具有统计学意义（P<0.05）。100μg/ml的PM_2.5处理细胞后,IL-1α和IL-1β表达水平高于IL-6和IL-8;炎症小体NLRP3、caspase-1和IL-18在100μg/ml的PM_2.5处理24、48 h后也呈现升高趋势,差异具有统计学意义（P<0.05）。结论夏季和冬季PM_2.5水溶性成分与总颗粒物可以抑制细胞活性,并且促使细胞炎症发生,冬季总颗粒物对细胞活性与炎症的影响更明显。更多还原

【Abstract】 Objective To understand the effects of water-soluble components and total particulate matter of PM_2.5 collected in summer and winter on cell viability and inflammation of BEAS-2B cells. Methods Four kinds of PM_2.5 components were obtained by extracting water-soluble components and total particulate matter from PM_2.5 collected in summer and winter respectively. The cell viability was determined by using CCK-8 kit after treated with PM_2.5 at the doses of 25,50,100,200 and400 μg/ml respectively. RNA was extracted and the levels of inflammatory cytokines IL-1α,IL-1β,IL-6,IL-8,and NLRP3 inflammatory body,caspase-1 and IL-18 were detected by q RT-PCR after 12 h-,24 h-and 48 h-treatment with 100 μg/ml PM_2.5,respectively. Results The cell viabilities increased after 12 h-treatment with water-soluble components and total particulate matter of PM_2.5 at some exposure doses,and as the exposure time increased,the cell viability was gradually inhibited,the difference was statistically significant（P<0.05）. The expression levels of IL-1α and IL-1β were higher than IL-6 and IL-8 in BEAS-2 B cells exposed to 100 μg/ml PM_2.5. Inflammatory body NLRP3 and its related molecules（caspase-1 and IL-18） also showed an increasing trend after PM_2.5 treatment at 100 μg/ml for 24 h and 48 h respectively,the difference was statistically significant（P<0.05）. Conclusion The water-soluble components and total particulate matter of PM_2.5 in summer and winter can inhibit cell viability and promote cell inflammation,and total particulate matter of PM_2.5 in winter has a greater adverse effects.更多还原