【摘要】 采用随机区组设计,分别对添加不同量的青霉菌和生物炭的砷污染土壤进行培养,通过测定土壤中的As³⁺、As⁵⁺及总砷含量,探究了青霉菌与生物碳复合修复对砷污染土壤中有效砷的钝化率及土壤中砷的价态转化的影响,同时对土壤中的微生物数量进行区系分析,建立了微生物数量与有效砷含量之间的关系.结果显示,随着青霉菌接菌量与生物炭施用量的增加,土壤中总砷含量不会发生变化,有效砷含量从17.74 mg·kg^-1下降到12.69 mg·kg^-1,有效砷的钝化率可达到27.6%左右.而两种价态的砷(As⁵⁺、As³⁺)之间没有发生转化,约27%的As⁵⁺会被青霉菌与生物炭固定,但As³⁺在土壤中的含量基本保持不变.在有效砷含量下降的同时,土壤中放线菌的含量基本不变,但土壤中细菌的总量有所上升.结果表明,青霉菌与生物碳复合修复可以降低有效砷的含量,并使砷污染土壤中的微生物环境有所改善,对砷污染土壤显示出较好的修复性能.更多还原

【Abstract】 In this paper, a randomized block design was used to cultivate with different amounts of penicillium and biochar for the remediation of arsenic-contaminated soil. By measuring the concentrations of As³⁺, As⁵⁺ and total arsenic in the soil, the effects of the penicillium and biochar on the passivation rate of arsenic and the conversion of the valence state of arsenic in the arsenic-contaminated soil were investigated. Meanwhile, the relationship between the amount of microorganisms and the effective arsenic content was established through the analysis of the number of microflora in the soil. The experimental results show that the total arsenic content in soil did not change with the increase of the applied amount of penicillium and biochar. The effective arsenic content decreased from 17.74 to 12.69 mg·kg^-1, and the passivation rate of effective arsenic reached up to 27.6%. However, there was no conversion between the two valence of arsenic(As⁵⁺, As³⁺), and about 27% of As⁵⁺ was fixed by penicillium and biochar, but the content of As³⁺ in soil remained unchanged basically. While the effective arsenic content decreased, the content of actinomycetes in soil remained basically the same, but the total amount of bacteria in soil increased. The results show that the combined remediation of penicillium and biochar could reduce the content of effective arsenic, improve the microbial environment in the arsenic-contaminated soil, and show good remediation performance to the arsenic-contaminated soil.更多还原