【摘要】 为探究施用有机肥对土壤中抗性基因和整合酶基因分布的影响及其与土壤环境因子的相关关系,利用高通量PCR技术监测了鸡粪有机肥施用120 d后土壤中四环素类抗性基因、大环内酯类抗性基因、整合酶基因和土壤理化性质的变化情况。研究结果表明,施肥土壤的电导率、pH、有机质含量均明显增加,而土壤氧化还原电位由217.27 mV降低到154.47 mV。施肥土壤中钾、氮、磷、铅、铜和锌含量均上升。施加鸡粪有机肥120 d后,土壤中tetM、tetQ、tetW、tetG和tetX 5种四环素类抗性基因相对丰度分别增加了2.90、0.97、6.80、0.98和0.94倍,而erm35、ermB、ermT、ermX和ermF 5种大环内酯类抗性基因相对丰度分别增加了0.98、136.68、0.95、2.89和2.89倍;但erm36相对丰度降低了0.75倍,其中施肥后土壤中ermB丰度最高而erm36丰度最低。施用鸡粪有机肥后,土壤中intI1和intI3的丰度分别降低了4.71×10-5和2.57×10-7,而intI2的丰度增加了3.74×10^-6。Network分析发现intI3与除ermB外的基因均呈显著负相关关系（R=-1.00,P<0.05）。更多还原

【Abstract】 In order to investigate the effects of the application of chicken manure organic fertilizer on the distribution of antibiotic resistance genes and integrase genes in soil and their correlations with environmental factors, high-throughput PCR was used to monitor the variations of tetracycline resistance genes, macrolactone resistance genes and integrase genes in soil, as well as the soil physicochemical properties, after 120 d of chicken manure organic fertilizer application. The results showed that the electrical conductivity（EC）, pH, and organic matter contents of manure applied soil increased, while oxidation-reduction potential（ORP） decreased from 217.27 mV to 154.47 mV. The contents of nitrogen, phosphorus, potassium, Cu, Zn, and Pb increased in soil. The relative abundances of five types of tetracycline resistance genes: tetM, tetQ, tetW, tetG and tetX,increased by 2.90, 0.97, 6.80, 0.98 and 0.94 times after 120 d of fertilization, respectively. The abundances of five types of macrolactone resistance genes: erm35, ermB, ermT, ermX, and ermF, increased by 0.98, 136.68,0.95, 2.89 and 2.89 times, but the abundance of erm36 decreased by 0.75 times, of which the highest abundance of ermB and the lowest one of erm36 occurred in the fertilized soils. After application of chicken manure organic fertilizer, the relative abundances of intI1 and intI3 in soil decreased by 4.71 × 10-5 and 2.57 × 10-7,respectively, while the relative abundance of intI2 increased by 3.74 × 10^-6. Network analysis showed that intI3 was negatively correlated with the genes except for ermB（R=-1.00, P < 0.05）.更多还原