【摘要】 为研究食源色氨酸添加量对不同密度下刺参生长和代谢酶活性的影响,在水温(17±0. 5)℃下将初始体质量(湿重)为(3. 5±0. 1) g的刺参(Apostichopus japonicus Selenka)饲养在40 L(53 cm×28 cm×34 cm)的玻璃水族箱中,密度分别为4(L组)、8(ML组)、16(MH组)和32(H组)头/40 L,分别投喂添加0%、1%、3%和5%色氨酸的饲料,饲养75 d,每个处理组设4个重复。试验测定了刺参体质量变化和代谢酶活性,结果显示:随着养殖密度增加,刺参生物量增加,刺参增重率下降。最大生物量为196. 1 g(3%色氨酸,H组),最低增重率为35. 9%(5%色氨酸,H组);与对照组相比,饲料中添加3%色氨酸处理组刺参生长更快;在各养殖密度下,添加3%色氨酸处理组的谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(MDH)和苹果酸脱氢酶(LDH)酶活力低于其他处理组。高密度(H组)下,添加3%色氨酸处理组的ALT、AST、MDH和LDH活力分别为5. 56 U/g prot、1. 85 U/g prot、0. 17 U/mg prot、64 U/g prot,低于对照组。研究表明,添加1%～3%食源色氨酸可以缓解高密度养殖对刺参的胁迫,提高刺参生长率。更多还原

【Abstract】 Apostichopus japonicus Selenka with initial body weight( wet weight) of( 3. 5 ± 0. 1) g was reared in a 40 L glass tank( 53 cm × 28 cm × 34 cm) at water temperature of( 17 ± 0. 5) ℃ at a density of 4 ind./tank( group L),8 ind./tank( group ML),16 ind./tank( group MH) and 32 ind./tank( groupH),and fed with diets supplemented with 0%,1%,3% and 5% Trp for 75 days with four replicates to investigate the effects of dietary Trp on growth and metabolic enzyme activities of A. japonicus Selenka reared at various densities. The body weight change and metabolic enzyme activities of A. japonicus Selenka were determined in the experiment. The results showed that the biomass of Apostichopus japonicus Selenka was increased and weight gain decreased as the stocking density increased. The maximum biomass was 196. 1 g( groupH,3%Trp),while the minimum weight gain was 35. 9( groupH,5% Trp). There was significantly higher growth rate in the A. japonicus Selenka fed with the diet containing 3% of Trp than that in the control group. The A.japonicus Selenka fed with the diet containing 3% of Trp had lower ALT activity,AST activity,MDH activity and LDH activity than that in other treatment groups at various stocking densities. The ALT activity,AST activity,MDH activity and LDH activity were 5. 56 U/gprot,1. 85 U/gprot,0. 17 U/mgprot and 64 U/gprot respectively when the diet containing 3% of Trp in H group,which were lower than those in the control group.The study showed that high stocking density produced stress effects on individual growth. Moreover,dietary supplementation of 1%-3% Trp significantly relieves crowding stress and improves the growth of A. japonicus Selenka.更多还原