【摘要】 以银腺杨无性系HYS-1组培苗叶片为材料,MS为基本培养基,通过单因素试验及正交试验L₁₆（4⁵）,探究不同浓度TDZ、6-BA以及不同生长素种类对银腺杨无性系HYS-1叶片再生体系建立的影响并筛选最佳培养基。结果表明:不定芽主茎高于1cm的芽占总芽数的比率随TDZ浓度的增加而降低。不定芽再生频率随6-BA浓度的增加而降低。在TDZ（0.05～0.10）mg/L+6-BA（0.50～1.50）mg/L的范围内,叶片通过愈伤组织间接诱导大量不定芽,无明显主茎;只有6-BA（0.50～1.50）mg/L时叶片直接诱导不定芽,且主茎明显。叶片基部是最佳不定芽诱导位置;TDZ 0.05mg/L+6-BA 0.30mg/L+NAA 0.05mg/L处理15d后即出现不定芽,且主茎较高,是银腺杨无性系HYS-1叶片诱导不定芽再生的最佳培养基。再生不定芽在1/2MS+0.50mg/L IBA生根培养基上,5d开始生根,生根率95%以上。更多还原

【Abstract】 Using tissue culture seedling leaves of Clone HYS-1 of Populus alba × P.glandulosa as material and MS as basic medium,through single factor test and orthogonal test L₁₆（4⁵）,exploring the influence of different concentrations of TDZ,6-BA and different kinds of growth elements on the establishment of leaf regeneration system of Clone HYS-1 of Populus alba × P.glandulosa was investigated and the best medium was selected.The results showed that the proportion of adventitious buds whose main stem was more than 1 cm to the total number of buds decreased with the increase of TDZ concentration. The frequency of adventitious bud regeneration decreased with the increase of 6-BA concentration. Within the range of 6-BA（0.50～1.50）mg/L+TDZ（0.05～0.10）mg/L,a large number of adventitious buds were indirectly induced from leaves through callus,but there was no obvious main stem;only 6-BA（0.50～1.50）mg/L could induce adventitious buds directly from leaves,and the main stem was obvious. The base of leaves was the best position for adventitious bud induction; adventitious buds appeared 15 days after treatment with 0.05 mg/L TDZ+0.30 mg/L 6-BA+0.05 mg/L NAA,and the main stem was higher,which was the best medium for leaf induction of adventitious bud regeneration of Clone HYS-1 of Populus alba × P.glandulosa. The regenerated adventitious buds began to take root on 1/2 MS+0.50 mg/L IBA rooting medium for 5 days,and the rooting rate was over 95%.更多还原