【摘要】 滨海湿地退化是全球关注的一个热点研究问题,其中湿地退化对土壤细菌群落结构和生态学功能的影响程度目前知之甚少。对北戴河大潮坪滨海湿地植被区和退化区土壤细菌多样性、环境因子和共现性关系的研究显示,滨海湿地植被区微生物香农指数显著高于退化区的,细菌OTU注释结果表明变形菌门(Proteobacteria)、浮霉菌门(Planctomycetes)、厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)、芽单胞菌门(Gemmatimonadetes)、酸杆菌门(Acidobacteria)和绿弯菌门(Chloroflexi)是主要的细菌类群,它们相对丰度共占群落的85%～95%。厚壁菌门的芽胞杆菌纲(Bacilli)在退化区丰度显著高于植被区的,浮霉菌门的Planctomycetia和Phycisphaerae在植被区的丰度明显高于退化区的。环境因子分析表明,退化区域微生物群落结构受土壤多环芳烃(PAHs)质量比和土壤含盐量的影响,其中土壤PAHs质量比高于1.273 mg/kg时,微生物多样性呈现下降趋势,土壤含盐量和细菌的丰富度呈负相关关系。属间共现性关系(Co-occurrence)研究表明随着滨海湿地的退化,土壤中固碳细菌的生态学功能逐渐增强。对滨海湿地退化影响土壤细菌群落结构和生态学功能的进一步认识,为滨海湿地生态修复和效果评价提供了科学依据。更多还原

【Abstract】 Coastal wetland degradation is a global ecological hot issue. However, little is known about the relationships between wetland degradation and structure and function of soil microbe. In the present study, we investigated the microbial diversity, environment factors and co-occurrence relationship in degradation areas and vegetation areas of Beidaihe Dachaoping coastal wetland. The Shannon index of the microbe in vegetation area was significantly higher than that of in degradation area of wetland, OTU annotation results indicated that Proteobacteria, Planctomycetes, Firmicutes, Actinobacteria, Gemmatimonadetes, Acidobacteria, and Chloroflexi dominated in the coastal wetland, and accounted for 85% to 95% of bacterial communities at the phylum level. In the class level, the abundance of Bacilli was significantly higher in degradation areas than that of vegetation areas. The abundance of Planctomycetia and Phycisphaerae both belonged to Planctomycetes was higher in the vegetation area than that of degradation area. The environment factors analysis indicated that the microbial community was affected by the concentration of polycyclic aromatic hydrocarbons(PAHs) and salinity in the degradation area. And when the concentration of PAHs was higher than 1.273 mg/kg, microbial diversity presented a downward trend. A negatively correlated relationship was observed between salinity and OTU richness. The co-occurrence relation analysis indicated that the function of carbon-fixing bacteria was enhanced in the degradation areas. This study showed that the degradation of coastal wetland changed the soil bacterial diversity and community structure, enhanced the understanding of soil bacteria community and function in vegetation and degradation areas, and provided a valuable reference for the ecological restoration and effective evaluation of coastal wetlands.更多还原