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一株发光细菌的分离鉴定及其对有毒物质的毒性响应

Isolation and Identification of A Luminous Bacterium and Its Toxicity Response to Hazardous Material

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【作者】 段效辉张群刘鹏王颖赵晗

【Author】 DUAN Xiaohui;ZHANG Qun;LIU Peng;WANG Ying;ZHAO Han;Technology Center of Yantai Entry-Exit Inspection and Quarantine Bureau;Technology Center of Ji′nan Entry-Exit Inspection and Quarantine Bureau;Technology Center of Weifang Entry-Exit Inspection and Quarantine Bureau;

【机构】 烟台出入境检验检疫局检验检疫技术中心济南出入境检验检疫局检验检疫技术中心潍坊出入境检验检疫局检验检疫技术中心

【摘要】 从新鲜海鱼肠道内分离获得1株发光细菌Fc-11-1,通过菌落形态特征、生理生化特征、16SrDNA序列分析及系统发育树分析对其进行鉴定,初步确定发光细菌Fc-11-1为Vibrio toranzoniae。从发光细菌Fc-11-1的生长和发光强度两方面,初步探索了发光细菌Fc-11-1对食品中5种常见有毒物质百菌清、呋喃唑酮、红霉素、氯霉素、阿维菌素的毒性响应。结果表明,发光细菌Fc-11-1对5种有毒物质均有不同程度的毒性响应,可用于食品中有毒物质残留的检测。

【Abstract】 We isolated a luminous bacterium Fc-11-1 from the intestinal tract of fresh marine fish.Moreover,we identified that the luminous bacterium Fc-11-1 belongs to Vibrio toranzoniae by colony morphology,physiological and biochemical characteristics,16 SrDNA sequencing analysis,and phylogenetic tree analysis.Furthermore,from the growth and luminescence intensity of the luminous bacterium Fc-11-1,we investigated the toxicity response of the luminous bacterium Fc-11-1 to five common hazardous materials,such as Chlorothaloni,Furazolidone,Chloramphenicol,Erythromycin,and Avermectin in food.The results show that the luminous bacterium Fc-11-1 has different degrees of toxicity response to five hazardous materials,which can be used for the detection of hazardous materials residues in food.

【基金】 国家重点研发计划(2017YFF0211103);原国家质检总局科技计划(2015IK200);烟台市重点研发计划(2018SFGY072)
  • 【文献出处】 化学与生物工程 ,Chemistry & Bioengineering , 编辑部邮箱 ,2019年08期
  • 【分类号】X836
  • 【网络出版时间】2019-08-07 16:42
  • 【被引频次】1
  • 【下载频次】170
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