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制马钱子及其制剂中士的宁和马钱子碱的含量测定
Determination of Strychnine and Brucine in Processed Strychnine Semen and Its Preparation
【摘要】 建立了超高效液相色谱(UPLC)测定制马钱子及其复方制剂痹祺胶囊、伸筋丹胶囊和晕复静片中士的宁和马钱子碱含量的方法。采用ZORBAX RRHD Eclipse Plus C18色谱柱;流动相A为甲醇,流动相B为含1. 2%乙酸和0. 15%三乙胺的水溶液;流动相A-流动相B(24∶76,V/V)等度洗脱,流速0. 16 mL/min;检测波长260 nm。士的宁和马钱子碱分别在0. 49~19. 6μg/mL和0. 51~20. 4μg/mL的范围内线性良好;加样回收率均大于80. 0%,RSD均不超过2. 0%。本法准确度高,重复性好,专属性强,适用于制马钱子及其制剂的含量测定。
【Abstract】 A method for determination of strychnine and brucine in processed strychnine semen and its preparations by ultra-performanceliquid chromatography( UPLC) was established. ZORBAX RRHDEclipse Plus C18 columnwas used,mobile phase Awas methanol and mobile phase B was solution of 1. 2% acetic acid and 0. 15% triethylamine. An isocratic elutionwas carried out using mobile phase A-mobile phase B( 24 ∶ 76, V/V). The flow rate was 0. 16 mL/min,detection wavelength was 260 nm. There was a good linear relationship of strychnine in the range of 0. 49 ~ 19. 6 μg/mL( R=0. 9997) and strychnine in the range of 0. 51~20. 4 μg/mL( R=0. 9998). The recoveries were above 80. 0%. RSD values were not more than 2. 0%. The proposed method was accurate and reproducible,which can be used for the assay of processed strychnine semen and its compound preparation.
- 【文献出处】 广州化工 ,Guangzhou Chemical Industry , 编辑部邮箱 ,2019年14期
- 【分类号】O657.72;R286.0
- 【被引频次】2
- 【下载频次】316