【摘要】 建立了超高效液相色谱（UPLC）测定制马钱子及其复方制剂痹祺胶囊、伸筋丹胶囊和晕复静片中士的宁和马钱子碱含量的方法。采用ZORBAX RRHD Eclipse Plus C₁₈色谱柱;流动相A为甲醇,流动相B为含1. 2%乙酸和0. 15%三乙胺的水溶液;流动相A-流动相B（24∶76,V/V）等度洗脱,流速0. 16 mL/min;检测波长260 nm。士的宁和马钱子碱分别在0. 49～19. 6μg/mL和0. 51～20. 4μg/mL的范围内线性良好;加样回收率均大于80. 0%,RSD均不超过2. 0%。本法准确度高,重复性好,专属性强,适用于制马钱子及其制剂的含量测定。更多还原

【Abstract】 A method for determination of strychnine and brucine in processed strychnine semen and its preparations by ultra-performanceliquid chromatography（ UPLC） was established. ZORBAX RRHDEclipse Plus C₁₈ columnwas used,mobile phase Awas methanol and mobile phase B was solution of 1. 2% acetic acid and 0. 15% triethylamine. An isocratic elutionwas carried out using mobile phase A-mobile phase B（ 24 ∶ 76, V/V）. The flow rate was 0. 16 mL/min,detection wavelength was 260 nm. There was a good linear relationship of strychnine in the range of 0. 49 ～ 19. 6 μg/mL（ R=0. 9997） and strychnine in the range of 0. 51～20. 4 μg/mL（ R=0. 9998）. The recoveries were above 80. 0%. RSD values were not more than 2. 0%. The proposed method was accurate and reproducible,which can be used for the assay of processed strychnine semen and its compound preparation.更多还原