【摘要】 白粉病是一种真菌病,对农作物造成普遍的威胁。目前国内外关于青稞抗白粉病的报道较多,但缺乏有关西藏青稞白粉病代谢组学的研究。采用广泛靶向代谢组学分析方法,研究了西藏青稞在白粉病感染后,在代谢产物水平的动态生理变化。结果表明:西藏青稞与白粉菌Blumeriagraminis(DC.)f.sp.hordei(Bgh)相互作用的过程中,全抗品种G7与感病品种Z13有不同的表型变化;广泛靶向代谢组共检测到300种已知和未知代谢产物,256个已知代谢物,全抗品种G7与感病品种Z13之间存在着52个代谢物的差异表达,分为15类。进一步分析表明,在青稞与Bgh相互作用的过程中,氨基酸、酚胺类物质的含量存在差异积累。本研究为青稞对白粉病应答过程中发生的动态生理变化提供新认识,将为西藏青稞抗性品种的开发提供新线索。更多还原

【Abstract】 Powdery mildew is a fungal disease that poses a widespread threat to crops. At present, there are many domestic and foreign reports relating to the resistance of Tibetan hulless barley to powdery mildew, but there is a lack of research on the metabolomics of Tibetan hulless barley powdery mildew. The dynamic physiological changes of Tibetan hulless barley at the level of metabolites after powdery mildew infection were studied by extensive targeted metabolomics analysis. The results showed that: during the interaction between Tibetan hulless barley and blumeria graminis(DC.) f.s. hordei(Bgh), there were different phenotypic changes between the resistant variety G7 and the susceptible variety Z13. A total of 300 known and unknown metabolites and 256 known metabolites were detected in the widely targeted metabolome, and 52 metabolites were differentially expressed between the resistant variety G7 and the susceptible variety Z13. It could be grouped into 15 categories. Further analysis showed that during the interaction between Tibetan hulless barley and Bgh,there were differences in the content of amino acids and phenolic amines. This study provided new understanding of the dynamic physiological changes occurring in the response process of hulless barley to powdery mildew. It will provide a new clue for the development of Tibetan hulless barley resistant varieties.更多还原