【摘要】 为了探索调节昆虫世代历期的相关基因,研究了菠萝洁粉蚧在转录水平上对温度的响应。组装后获得49 898个Unigenes,其平均长度为1 007 bp;其中有15 015 (30.10%)个Unigenes被成功注释到Nr、SwissProt、KOG和KO等功能数据库中。DEG分析表明,7 960个Unigenes被诱导差异表达,其中MIX21相对于MIX27表达量上调的有5 080个,下调的有2 880个。差异基因代谢通路分析表明,差异表达基因中涉及长寿调节途径(Longevity regulating pathway)的有3个通路,其中21℃饲养下的菠萝洁粉蚧种群胰岛素/IFG-1信号通路关键因子DAF-2、雷帕霉素靶标TOR信号通路关键因子RSK-1和S6K显著下调,相关因子被抑制表达,很好地解释了其世代发育历期比27℃饲养下长的现象。21℃饲养下的菠萝洁粉蚧种群HSP60较27℃饲养下的显著上调,而HSPs有上调也有下调,显示热休克蛋白HSP对温度的反应呈现一定的多样性、复杂性。这些发现将有助于今后昆虫寿命分子调节机制的研究,DAF-2、S6K、RSK-1等相关基因未来或将应用于调节一些经济昆虫的生长周期。更多还原

【Abstract】 In order to explore the genes related to generation period of the insect, we studied the transcriptomic response of D. brevipes to temperature. We obtained 49 898 assembled unigenes, with average length of 1 007 bp,15 015(30.10%) of which were annotated in Nr, SwissProt, KOG or KO cDNA libraries. The analysis of differentially expressed genes(DEG) showed that 7 960 unigenes were differentially expressed, of which 5 080 unigenes were up-regulated and 2 880 unigenes were down-regulated significantly compared to MIX27 population. As for KO annotation, there were three corresponding pathways longevity regulating. The key factors for signaling pathways of insulin/insulin-like growth factor 1 and target of rapamycin, DAF-2, RSK-1 and S6 K of MIX21 population were down-regulated significantly, their expression were suppressed, which was probably the right explanation for its longer generation period and longevity compared to MIX27 population. The relevant genes for heat-shock protein were complicated, with up-regulated significantly of HSP60 and up-regulated or down-regulated significantly of HSPs compared to MIX27 population. The founding contributed to explore the molecular regulating mechanism of longevity of insect, these related genes, DAF-2, S6 K and RSK-1 might be applied to regulate the growth period of economic insects in the future.更多还原