【摘要】 以萝卜籽为原料,对其富硒培育条件进行优化。选择影响富硒萝卜苗发芽率及硒含量的Na₂SeO₃浓度、浸泡温度、浸泡时间三个因素进行单因素实验。并在此基础上,采用Box-Behnken试验设计原理,以萝卜苗发芽率为因变量,硒含量为伴生变量,对富硒萝卜苗发芽工艺进行优化,实验表明:Na₂SeO₃浓度、浸泡温度、浸泡时间对富硒萝卜苗发芽率的影响大小依次为:Na₂SeO₃浓度>浸泡时间>浸泡温度。最佳处理参数为:Na₂SeO₃浓度80 mg/L、浸泡温度24℃、浸泡时间8 h,在此条件下富硒萝卜苗发芽率为（96.06±0.12）%,相应的硒含量为（4.453 6±0.135 2）μg/g,与理论值4.450 4μg/g基本吻合。该研究采用Box-Behnken响应面法进行富硒萝卜苗发芽工艺优化,获得了富硒萝卜苗最佳培育条件,该工艺稳定可行,预测性良好,为富硒萝卜苗的生产开发提供参考依据。更多还原

【Abstract】 This paper optimize the cultivation conditions of selenium-enriched sprouts by taking selenium-rich liquid fertilizer as turnip sprouts source. Na₂SeO₃ concentration, soaking temperature and soaking time were selected as the factors in optimization, the effect of different factors to the germination rate and selenium content of selenium-enriched turnip sprouts were studied. The optimum processing conditions for selenium-enriched turnip sprouts were determined by single factor experiment and box-behnken design. The experiments showed that the effects of Na₂SeO₃ concentration, soaking temperature and soaking time on germination rate of selenium-riched turnip sprouts were Na₂SeO₃ concentration>soaking time>soaking temperature. The best conditions were: Na₂SeO₃ concentration of 80 mg/L, soaking temperature 24℃, soaking time 8 h, under the above conditions, the germina tion rate was（96.06±0.12）%, the selenium content of selenium-enriched turnip sprouts was（4.453 6±0.135 2） μg/g,consistent with the theoretical value of 4.450 4 μg/g. In this study, box-behnken response surface method was used to optimize the germination process of selenium-enriched turnip sprouts, obtaining the best breeding technology.The technology is stable, feasible and predictable, which provides reference for the production and development of selenium-riched turnip sprouts.更多还原