【摘要】 植物BBX蛋白作为转录因子参与植物多种生命活动。为了研究草莓中BBX家族成员的基因功能,在前期分析转录组数据得到一个响应不同光质处理的草莓BBX家族成员。通过同源克隆的方法从‘红颜’草莓中分离克隆得到一个BBX蛋白编码c DNA序列,并对其进行生物信息学分析、基因表达分析、亚细胞定位与酵母转录激活分析。结果表明:克隆得到一条1 403 bp的cDNA序列,含有编码蛋白长度为447个氨基酸的CDS序列,编码蛋白与拟南芥(Arabidopsis thaliana) AtBBX15蛋白同源,命名为FaBBX15。蛋白质预测分子量49 831.42 Da。FaBBX15蛋白质含有1个B-box保守结构域和1个CCT保守结构域,属于BBX蛋白家族第3亚家族成员。定量PCR分析表明FaBBX15基因表达受到红光与蓝光抑制。FaBBX15蛋白质在烟草表皮亚细胞中定位于细胞核,在酵母细胞中具有转录激活能力,具有典型的转录因子特征。本研究结果可为进一步揭示FaBBX15蛋白在光信号转导中的的基因功能及分子机理提供理论参考。更多还原

【Abstract】 As transcript factors, BBX proteins participate in various physiologically processes of plant. In order to investigate the gene function of BBXs in strawberry, a member of BBX family in strawberry responding to different light quality treatments was obtained by analyzing transcriptome data in advance. A BBX protein encoding cDNA sequence was isolated and cloned from ’Benihhope’ strawberry by homologous cloning, and its bioinformatics analysis, gene expression analysis, subcellular localization and yeast transcription activation analysis were carried out. The results showed that a 1 403 bp c DNA sequence was cloned, containing a CDS sequence with 447 amino acids. The coding protein was homologous to Arabidopsis thaliana AtBBX15 protein and named FaBBX15.The predicted molecular weight was 49 831.42 Da. Fa BBX15 protein contained one B-box conserved domain and one CCT conserved domain and belonged to the third subfamily of BBX protein family. Quantitative PCR analysis indicated that FaBBX15 gene expression was inhibited by red and blue light. FaBBX15 protein was located in the nucleus of tobacco epidermal subcells and has transcriptional activation ability in yeast cells with typical transcription factor characteristics. The result of this study might provide a theoretical reference for further revealing the gene function and molecular mechanism of Fa BBX15 protein in light signal transduction.更多还原