【摘要】 植物CBL家族基因在逆境胁迫应答中具有重要功能,但在马铃薯中鲜有报道。本研究通过生物信息学方法在马铃薯基因组中筛选出13个CBL基因,并鉴定了这些基因的染色体分布、理化性质、遗传进化、序列结构和顺式作用元件等。结果发现,马铃薯CBL基因在染色体上的分布是不均匀的,编码区长度在642～774 bp之间,外显子数除了StCBL1只含有一个以外,其余CBLs的外显子数多为7～9个;在遗传进化上,马铃薯CBL可分为两大组,且多与番茄CBL同源;通过对其编码蛋白序列进行比对,发现每个CBL均具有4个变异程度不等的EF-hand结构域;其含有的作用位点类型也有所差异,StCBL2/StCBL8/StCBL9的N端不含有常规的豆蔻酰化位点,其C端的核心基序FPSV中,缺失了可被CIPK磷酸化的关键丝氨酸残基;在马铃薯CBL基因的上游序列中发现了可以响应不同植物激素以及逆境胁迫的顺式作用元件。本研究对马铃薯CBL家族进行了鉴定与初步分析,可为进一步研究马铃薯CBL基因功能提供理论依据。更多还原

【Abstract】 Plant CBL family genes have important functions in stress response, but they are rarely reported in potatoes. In this study, 13 CBL genes were screened in the potato genome by bioinformatics methods, and the chromosome distribution, physicochemical properties, genetic evolution, sequence structure and cis-acting elements of these genes were identified. The results showed that the distribution of CBL gene on the chromosome of potato was not uniform. The length of the coding region was between 642 bp and 774 bp, and the number of exons for StCBL1 was only one, while for others was 7 to 9. In genetic evolution, potato CBL could be divided into two groups, and most of them were homologous to tomato CBL. By comparing the sequence of its coding protein,it was found that each CBL had four EF-hand domains with varying degrees of variation. They contained different types of action sites. The N-terminus of StCBL2/StCBL8/StCBL9 did not contain a conventional myristoylation site,and the C-terminal core FPSV-motif lacked the key serine residues, which could be used for phosphorylation by CIPK(CBL-interacting protein kinases). Cis-acting elements responsive to different plant hormones and stress were found in the upstream sequence of potato CBL gene. In this study, potato CBL family was identified and preliminarily analyzed, which could provide theoretical basis for further study of potato CBL gene function.更多还原