【摘要】 为了挖掘参与槟榔主要次生代谢产物生物合成与积累的关键酶基因,并明确其组织特异性,本研究以‘热研1号’槟榔雄花、雌花、种子、果肉为试验材料,用Illumina HiSeq TM 2000分别进行转录组测序,共获得265 505 184个Clean reads片段,组装后与Nr、KOG、KEGG、Swiss-Prot四大数据库进行比对,共有78 798个Unigenes被四大数据库注释,其中NR共注释35 467个Unigenes,对比后发现槟榔与油棕、海枣等具有一定的相似性;Swiss-prot共注释23 722个Unigenes;KOG共注释20 499个Unigenes,根据功能将槟榔转录组中的Unigene分为26类;KEGG共注释20 499个Unigenes,有10 009个Unigenes被四大数据库同时注释。槟榔转录组数据库的建立,可以有效地为解决槟榔次生代谢调控方面研究空白及活性成分合成和积累的代谢途径尚未明确的问题给予强大数据支持。该研究所得结果有助于槟榔药用成分的大量生产,为槟榔药用价值的开发利用提供依据。更多还原

【Abstract】 In order to find the key enzyme genes involved in the biosynthesis and physiological processes of the main secondary metabolites of Areca catechu, and to clarify their expression level and tissue specificity, this study used ’Reyan No.1’ Areca as the material to sequence the areca sample transcriptome with Illumina HiSeqTM2000.A total of 265 505 184 Clean reads fragments were obtained. After assembly, the areca transcriptome All-Unigene was annotated to the Nr, KOG, KEGG, and Swiss-Prot databases, and the number of Unigenes annotated in each database was counted. A total of 78 798 Unigenes were used in the four databases. 35 467 Unigenes were annotated to NR database. After comparison, it is found that areca has similarity with Elaeis guineensis and Phoenix dactylifera. A total of 23 722 Unigenes were annotated to Swiss-prot database; 20 499 Unigenes were annotated to KOG database. According to the function of the areca transcriptome, Unigenes is divided into 26 categories; 20 499 Unigenes were annotated to KEGG pathway, and 10 009 Unigenes are simultaneously annotated by four major databases. The establishment of the Areca catechu transcriptome database can effectively provide strong data support for solving the problems of the blank research on the regulation of Areca catechu secondary metabolism and the unclear metabolic pathways of the synthesis and accumulation of active components. The results of this study are helpful to the mass production of the medicinal components of areca nut and provide the basis for the exploitation and utilization of the medicinal value of Areca catechu.更多还原