【摘要】 研究宽频电磁脉冲(Wide-band electromagnetic pulse, WEMP)辐照对正常细胞的损伤作用,寻找辐照参数与细胞凋亡之间的作用规律,为探索WEMP辐照损伤机理模型提供实验依据。利用Cell Counting Kit-8检测WEMP辐照后细胞的活力变化,采用流式细胞术分析细胞凋亡损伤情况,利用统计分析和数据拟合的方法探讨WEMP场强、脉冲个数与细胞凋亡之间的作用规律。结果表明,在本实验条件下,场强与脉冲个数都是引起人羊膜上皮细胞(AECs)凋亡率增加的因素,其中,电场强度为主要因素,脉冲个数为次要因素,两者之间存在交互作用。细胞凋亡率与电场强度之间呈现出Logistic函数规律。更多还原

【Abstract】 The purpose of this study was to investigate the relationship between cell apoptosis and wide-band electromagnetic pulse(WEMP) irradiation parameters and to provide experimental data to establish the biophysics damage model for WEMP. Cell counting kit-8 was used to detect the cell viability changes after WEMP irradiation and flow cytometry assay was used to detect cell apoptosis after irradiation under different WEMP irradiation parameters. Under experimental conditions, field strength and number of pulses were observed to be the main factors that increased the apoptotic rate of human amniotic epithelial cells. Specifically, electric field intensity and number of pulses were determined to be the primary and secondary factors, respectively. The two factors were confirmed to interact with each other, that is, a logistic function was found to exist between the apoptotic rate and electric field strength.更多还原