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应用体外仿生消化法对华贵栉孔扇贝体内镉进行生物可给率研究

Bioaccessibility of cadmium in Chlamys nobills by in vitro bionics digestion model

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【作者】 杨妙峰郑盛华席英玉罗冬莲钟硕良

【Author】 YANG Miaofeng;ZHENG Shenghua;XI Yingyu;LUO Donglian;ZHONG Shuoliang;Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian;Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources;

【机构】 福建省水产研究所福建省海洋生物增养殖与高值化利用重点实验室福建省海洋生物资源开发利用协同创新中心

【摘要】 镉(Cd)是可食用贝类体内的主要污染物之一,为了更科学有效地研究贝类Cd的食用风险,以对Cd生物富集能力强的浅海贝类——华贵栉孔扇贝(Chlamys nobills)为研究对象,采用模拟胃肠道物理化学环境的体外仿生消化法,测定扇贝体内Cd的生物可给率。结果表明:华贵栉孔扇贝体内Cd的可给率范围在36.5%~63.3%之间,均值47.9%,消化食糜中Cd的主要形态为MT-Cd和GSH-Cd。扇贝各组织Cd的生物可给率大小依次为性腺(51.2%)、闭壳肌(44.9%)、内脏(40.6%)、鳃瓣/外套膜(37.4%),性腺质量分数与可给率呈显著负相关(R=-0.840,P<0.05),内脏质量分数则与可给率呈极显著正相关(R=0.996,P<0.01)。鳃瓣/外套膜和闭壳肌是扇贝消化食糜中Cd含量的主要来源(占63.5%),内脏对消化食糜中Cd的贡献率则高于其相应的质量分数,食用前若去除内脏,可以减少约25%Cd的膳食摄入量。

【Abstract】 Cadmium was one of the easiest accumulated environmental pollutants,and Chlamys nobills which belonged to the kind of shellfish in shallow sea and had significant bio-accumulation ability for Cd,was taken as an example to study the dietary intake risk scientifically and effectively.And then the method of in vitro digestion,which could simulate the physical and chemical environment of the gastrointestinal tract,was used to obtain the bioaccessibility of Cd in C.nobills for dietary intake.The result showed that the range of Cd bioaccessibility in C.nobills was from 36.5 to 63.3%(mean to 47.9%)and the main forms of Cd in chyme were MT-Cd and GSH-Cd.The Cd bioaccessibility of different tissues was in the order as sex gland(51.2%),adductor muscle(44.9%),viscera(40.6%)and gill lamella/mantle(37.4%).It also seemed that there was significant negative correlation between the mass fraction of sex gland and its bioaccessibility(R=-0.840,P<0.05),while a extremely significant positive correlation could be found between the mass fraction of visceral and its bioaccessibility(R=0.996,P<0.01).And the content of Cd in chyme mainly came from the tissue of gill lamella/mantle and adductor muscle(up to 63.5%).Because of the higher Cd contribution of viscera in the chyme than its corresponding mass fraction,the dietary intake risk of Cd might be reduced by a quarter if this tissue was removed before eating.

【关键词】 华贵栉孔扇贝生物可给率
【Key words】 Chlamys nobillscadmiumbioaccessibility
【基金】 福建省省属公益类科研院所基本科研专项(闽海渔科2017R003-9);闽台重要海洋生物资源高值化开发技术公共服务平台(2014FJPT01);厦门南方海洋研究中心项目(14PZY017NF17)
  • 【文献出处】 渔业研究 ,Journal of Fisheries Research , 编辑部邮箱 ,2019年05期
  • 【分类号】S917.4;X503.225
  • 【下载频次】89
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