【摘要】 将80只小鼠随机分成5组（空白对照组、模型组及牛至香酚低、中、高剂量组）,牛至香酚低、中、高剂量组小鼠分别按25、50、100 mg·kg^-1的剂量灌胃牛至香酚,空白对照组、模型组灌胃相同体积的橄榄油,每日1次,连续14 d.第15天时,牛至香酚试验组、模型组小鼠腹腔注射8 mg·kg^-1脂多糖（LPS）,6 h后采集脾脏组织,用qRT-PCR检测小鼠脾脏细胞因子（TNF-α、IFN-γ、IL-6、IL-10、IL-1β）、NF-κB及核转录因子（T-bet、GATA-3）mRNA的表达量,研究牛至香酚对LPS所致的免疫应激小鼠脾脏细胞因子及核转录因子mRNA表达的影响.结果表明:与空白对照组相比,模型组小鼠TNF-α、IFN-γ、IL-6、IL-10、IL-1β、NF-κB、T-bet mRNA的表达量提高（P<0.01）,GATA-3 mRNA的表达量降低（P<0.01）;与模型组相比,牛至香酚低、高剂量组IL-1β mRNA的表达量降低（P<0.01）,低、中、高剂量组IL-6、TNF-α mRNA的表达量降低（P<0.01、P<0.05）,低、中、高剂量组IFN-γ mRNA的表达量极显著或显著降低（P<0.01或P<0.05）,高剂量组NF-κB mRNA的表达量降低（P<0.05）,低、中、高剂量组T-bet mRNA的表达量降低（P<0.05）,中剂量组GATA-3 mRNA的表达量提高（P<0.05）.由本试验结果可知,牛至香酚通过提高或降低小鼠脾脏细胞因子、核转录因子的表达,调节免疫应激小鼠的免疫功能.更多还原

【Abstract】 To investigate whether oregano phenol promotes the expression of cytokines and positively regulate the nuclear factor mRNA in mice with immune stress arose from lipopolysaccharide（LPS）, 80 mice were randomized into 5 groups, namely 3 experimental groups lavage with 25, 50, and 100 mg·kg^-1 oregano phenol, respectively, 1 model group lavage with same amount of olive oil and last control group fed with water. After continuous gavage for 14 d, mice from 3 experimental groups and the model group were intraperitoneally injected 8 mg·kg^-1 LPS, with the control group injected physiological saline solution. After 6 h, spleen were sampled and the expressions of spleen cytokines（TNF-α, IFN-γ, IL-6, IL-10, IL-1β）, NF-κB and nuclear transcription factors（T-bet, GATA-3） mRNA were detected by qRT-PCR. The results showed that the expression levels of TNF-α, IFN-γ, IL-6, IL-10, IL-1β, NF-κB and T-bet mRNA were significantly elevated in the model group than the control（P<0.01）, while the expression of GATA-3 mRNA was significantly decreased（P<0.01）. Compared with the model group, the expression levels of IL-1β mRNA in low-and high-dose groups dropped significantly（P<0.01）. The expressions of IL-6（P<0.01） and TNF-α mRNA（P<0.05） in the experimental groups went down. The expression of IFN-γ mRNA decreased or significantly decreased in the experiment groups（P<0.01 or P<0.05）. The expression of NF-κB mRNA decreased only in the high-dose experimental group（P<0.05）, while the expression of T-bet mRNA decreased in all experiment groups（P<0.05）. And the expression of GATA-3 mRNA only increased in the medium-dose experiment group（P<0.05）. To sum up, oregano phenol could regulate the immune function of mice with immune stress by adjusting the expression of cytokines and transcription factors in mouse spleen.更多还原