【摘要】 为探究转BpTCP7基因白桦的耐盐碱能力,对前期获得的4个转BpTCP7白桦株系（TCP7-1,TCP7-2,TCP7-3,TCP7-4）及非转基因对照株系进行不同浓度的NaHCO₃处理,通过生长量、SOD、MDA及脯氨酸测定,分析转基因株系的耐盐碱能力。结果表明:低浓度的NaHCO₃（0.2%）处理后转基因及对照株系差别不明显,但中、高浓度的NaHCO₃（0.4%、0.8%）处理后,对照株系的细胞膜损伤程度明显高于大部分转基因株系,而渗透调节物及活性氧清除酶类的积累明显低于大部分转基因株系;大部分转基因株系的高生长也高于对照株系。说明BpTCP7基因一定程度上提高了植物的抗盐碱能力。更多还原

【Abstract】 To explore the salt tolerance of BpTCP7 transgenic lines,in this study,4 transgenic lines（TCP7-1,TCP7-2,TCP7-3,TCP7-4）and 1 non-transgenic line were treated with different concentrations of NaHCO₃.The salt tolerance of transgenic lines was analyzed by growth,SOD,MDA and proline determination.The results showed that the difference between transgenic and control lines of salt treated with low concentration is not obvious,but the cell membrane damage of salt treated with medium and high concentration was significantly higher than that of most transgenic lines,while the accumulation of osmotic regulators and active oxygen scavenging enzymes was significantly lower than that of most transgenic lines.The high growth of most transgenic lines was higher than that of the control line.It is indicated that the BpTCP7 gene improves the ability of salt tolerance of plants to some extent.更多还原