【摘要】 目的探讨切伦科夫光学显像在棕色脂肪组织代谢研究中的应用价值,并评估早衰小鼠棕色脂肪组织的代谢活性变化。方法以早衰小鼠为研究模型,采用切伦科夫光学成像动态监测早衰小鼠棕色脂肪组织代谢活性的变化,以¹⁸F-FDG PET/CT成像为参照;通过检测棕色脂肪组织活性分子标志物解偶联蛋白酶1（uncoupling protein 1,UCP1）的转录和蛋白表达水平评估早衰小鼠棕色脂肪组织衰老情况。结果 4～12周龄时,早衰小鼠棕色脂肪组织SUV_max及切伦科夫光学显像光强度值未见明显变化（P>0.05）,14周龄时早衰小鼠棕色脂肪组织PET/CT成像最大摄入值(SUV_max)、切伦科夫光强度值较12周龄时均显著下降（P<0.01）。分子水平上早衰小鼠UCP1的mRNA水平于12周龄开始下降（P<0.05）,而蛋白表达水平在14周龄时显著下降（P<0.01）,与显像结果一致。结论切伦科夫光学显像能够有效评估小鼠棕色脂肪组织代谢活性;早衰小鼠14周龄即出现棕色脂肪组织代谢活性的减退。更多还原

【Abstract】 Objective To study the changes in the metabolic activity of brown adipose tissues in a mouse model of progeria using Cerenkov luminescence imaging. Methods18 F-FDG PET/CT imaging and Cerenkov luminescence imaging were used to dynamically monitor the metabolic activity of the brown adipose tissue in progeria mice. The ageing of the brown adipose tissue was evaluated by detecting the transcriptional and protein expression levels of uncoupling protein 1( UCP1),a molecular marker of brown adipose tissue activity. Results At 4 to 12 weeks of age,the progeria mice showed no obvious changes in the SUVm ax and Cerenkov luminescence imaging light intensity in the brown adipose tissue( P>0.05). At 14 weeks of age,the SUVm ax and Cerenkov imaging light intensity in the brown adipose tissue were decreased significantly in progeria mice( P<0.01). At the molecular level,the mRNA expression level of UCP1 began to decrease in the progeria mice at 12 weeks( P<0.05),while UCP1 protein expression level decreased significantly at 14 weeks of age( P < 0. 01),which were consistent with the results of the imaging studies. Conclusion Cerenkov luminescence imaging allows efficient assessment of the changes in the metabolic activity of the brown adipose tissue in mice. In progeria mice,the metabolic activity of the brown adipose tissue becomes lowered as early as14 weeks of age.更多还原