【摘要】 以硫化亚铁（FeS）为电子供体的自养反硝化反应对水体中硝酸盐的去除有重要贡献.以菌株Thiobacillus（T.）denitrificans ATCC 25259为对象,首次研究了脱氮硫杆菌以FeS为底物的自养反硝化过程.结果表明,以FeS作为唯一电子供体时,T.denitrificans可以将NO^-₃-N(30 mg·L^-1)彻底还原为N₂.同时,FeS中的硫元素经自养反硝化过程转化为SO■,而铁元素与培养基中PO■反应生成沉淀物Fe₃（PO₄）₂·8H₂O.通过对相关数据拟合发现自养反硝化过程遵循零级反应动力学（R²>0.93）,随着FeS加入量的增大,NO^-₃和NO^-₂的还原速率均增大,但NO^-₃的还原速率增大更多,使中间产物NO^-₂的累积量增大.更多还原

【Abstract】 The autotrophic denitrification reaction with ferrous sulfide（FeS） as the electron donor plays a significant role in nitrate removal in water body. The strain Thiobacillus（T.） denitrificans ATCC 25259 is novelly applied to investigate the process of autotrophic denitrification with FeS as substrate. The results exhibit that NO^-₃-N(30 mg·L^-1) is finally reduced to N₂ by T. denitrificans utilizing FeS as the sole electron donor. The elemental sulphur in FeS is converted to SO■ via autotrophic denitrification, whereas the elemental iron is precipitated with PO■ as the form of Fe₃（PO₄）₂·8 H₂O. Besides, according to the fitted curves, this autotrophic denitrification process is demonstrated to accord with the zero-order kinetics（R²>0. 93）. As the rising amount of FeS, both the reduction rates of NO^-₂ and NO^-₃ increase. Additionally, the reduction rate of NO^-₃ grows even more sharply, which accounts for the improved accumulation concentration of NO^-₂ as intermediates.更多还原