【摘要】 目的用生物信息学方法筛选内皮祖细胞(EPCs)外泌体miRNA用于调节血管生成的医学科学研究。方法从现有数据库中筛选人内皮祖细胞外泌体miRNAs,同时筛选与血管生成相关miRNAs,取两组miRNAs的交集,从而获得内皮祖细胞外泌体中与血管生成相关的候选miRNAs分子。结果通过实验数据分析和文献筛选获得的内皮祖细胞外泌体miRNAs分别为160个和50个,与血管生成相关miRNAs分子约600个;分别取频次较高的前20个,最终获得9个在内皮祖细胞外泌体中可能高表达且与血管生成相关的miRNAs:miR-126、miR-21、miR-221、miR-92a、miR-199a、miR-210、miR-214、miR-155、miR-146a。结论基于数据分析和文献筛选的生物信息学可简单而可靠地筛选目标miRNA进行后续研究,有较高推广应用价值。更多还原

【Abstract】 Objective By taking usage of bioinformatics screening methods,this medical research aimed at exploring how the miRNAs in endothelial progenitor cell exosomes relate to the regulation of angiogenesis. Methods miRNAs in endothelial progenitor cells exosomes and angiogenesis-related miRNA was intersected from the existing database to obtain the candidates of miRNA molecules related to angiogenesis in endothelial progenitor exosomes. Results 160 and 50 miRNAs in endothelial progenitor cell candidates were obtained through experimental data analysis and literature searching respectively. 600 candidates of angiogenesis-related miRNAs were obtained through literature searching; the top 20 with the highest frequency were selected out. Finally,9 miRNA candidates(miR-126,miR-21,miR-221,miR-92 a,miR-199 a,miR-210,miR-214,miR-155,miR-146 a) that may be highly expressed in endothelial progenitor exosomes and associated with angiogenesis were obtained for the following research. Conclusions Based on data analysis and literature searching,bioinformatics could screen out the target miRNAs for follow-up studies easily and reliably,it is worthy to be widely applied and popularized.更多还原