【摘要】 为了明确白腐真菌偏肿革裥菌（Lenzites gibbosa）降解双偶氮染料刚果红过程中细胞色素P450基因的功能,采用高通量测序技术对刚果红染料处理0、3、7、10 h下的菌丝样本进行转录组测序,以比对到参考基因组上的每个基因的reads数即Count值作为表达量值,根据基因在对照组（0 h）和染料处理组（3、7、10 h）中表达量的变化,用DESeq_EBSeq软件进行样品组间的差异基因表达分析和差异基因筛选,对差异表达基因（DGEs）在eggNOG、GO和KEGG等数据库中进行功能注释与通路富集分析,对筛选出的相关差异基因在NCBI上进行DNA序列比对,并与相关参考文献进行比较分析。结果表明:在转录组所有的DGEs中,搜索出11个可能影响L. gibbosa降解刚果红过程的细胞色素P450基因,其中在CK vs GGH1（0 vs 3 h）中未筛选出细胞色素P450差异基因,在CK vs GGH2（0 vs 7 h）中筛选出3个差异基因,在CK vs GGH3（0 vs 10 h）中筛选出8个差异基因,表明在L. gibbosa对刚果红染料脱色降解过程中细胞色素P450基因逐步参与了降解脱色过程。在10 h时,被注释到木质素降解关键通路ko 01220（芳香族化合物降解）中的gene₂5488表现出差异表达。从11个细胞色素P450基因的差异表达中可以探究并揭示出一些潜在的生物学规律,最终结果表明L. gibbosa的细胞色素P450基因与双偶氮类染料刚果红的降解脱色有一定的关系。更多还原

【Abstract】 In order to clarify the function of cytochrome P450 genes from white rot fungus Lenzites gibbosa growing under the degradation of diazo dye Congo red,the transcriptomes were constructed on mycelia samples treated with Congo red at 0,3,7,and 10 h using high-throughput sequencing technology. The number of reads of each gene aligned to the reference genome,i.e. Count value,was used as the value of expression quantity,DESeq_EBSeq software was used to analyze the expression of differential genes and screen the differentially expressed genes（ DEGs） according to the changes of gene expression quantity between the control group（ 0 h） and the dye treatment group（ 3,7,and 10 h）. The DEGS were functionally annotated in eggN OG,GO,KEGG,and other databases,and significantly enriched pathways of DEGs were analyzed. The screened DEGs were alligned on NCBI for DNA sequences and compared with relevant references. The 11 cytochrome P450 genes that may affect the degradation process of Congo red by L. gibbosa were searched out from all the DGEs of transcriptomes,among them,no differentially expressed cytochrome P450 genes were screened in CK vs GGH1（ 0 vs 3 h）,3 cytochrome P450 genes were screened in CK vs GGH2（ 0 vs 7 h）,and 8 cytochrome P450 genes were screened in CK vs GGH3（ 0 vs 10 h）,indicating that cytochrome P450 genes are gradually involved in the degradation and decolorization process of Congo red by L. gibbosa. At 10 h,gene₂5488 annotated into the key pathway of lignin degradation ko01220（ degradation of aromatic compounds） showed differential expression. Some potential biological regulations can be explored and revealed from the differential expression of 11 cytochrome P450 genes. The final results showed that the cytochrome P450 gene of L. gibbosa have some relationship with the degradation and decolorization of the diazo dye Congo red.更多还原