【摘要】 将100只21日龄雏鸭随机分为AIV感染组（I）和对照组（C）,应用免疫学和细胞培养及MTT等方法动态检测AIV感染雏鸭血液红细胞免疫黏附能力、红细胞免疫自身调控能力以及红细胞促外周血淋巴细胞增殖能力。结果表明,雏鸭感染AIV后1～14 d,红细胞C_3b受体花环（E-CRR）率明显低于对照组,而红细胞免疫复合物花环（E-ICR）率明显高于对照组;AIV感染雏鸭后3～21 d,红细胞C_3b受体花环促进率（RFER）显著低于对照组,且红细胞C_3b受体花环抑制（RFIR）率显著高于对照组;AIV感染雏鸭的红细胞对血液T、B淋巴细胞增殖促进能力明显低于对照组。表明AIV感染引起的雏鸭红细胞免疫黏附功能、自身调控能力和T、B淋巴细胞增殖能力下降,与AIV感染导致雏鸭外周血免疫功能降低密切相关。更多还原

【Abstract】 In this study, one hundred 21-day-old ducklings were randomly assigned into two groups: AIV infection group（I） and un-infection group（C）. Erythrocyte immune adherence function,erythrocyte immune self-regulation ability and regulative ability of erythrocyte acting on lymphocyte proliferation were performed to figure out the mechanism of duckling immune pathogenesis with MTT method. The results showed that erythrocyte C_3b receptor rosette（E-CRR）ratio of duckling infected with AIV was significantly decreased than that AIV un-infection ducklings in 1-14 d. erythrocyte immune complex rosette（E-ICR） ratio of AIV infection group was significantly higher than that of control group in1-14 d; the immune enhancing factor of AIV infection ducklings were lower than that in control group from 3-21 d, while the immune inhibition factor of AIV infection group were higher from 3-21 d than that in control group; Besides, the ability of T, B lymphocytes proliferation in the AIV infection group was obviously lower than that in control group. Therefore, this study illustrated that the AIV infectioninduced declines of erythrocyte immune adherence function, self-regulation ability and T, B lymphocyte proliferation ability in ducklings are closely related to erythrocyte immune.更多还原