【摘要】 肠道微生物在宿主的生理活动、免疫调节以及健康方面均有重要的作用。尽管已有报道表明高原鼠兔肠道菌群和食物、环境有关,但对于野生和室内饲养条件下,取食不同食物的高原鼠兔肠道菌群是否存在差异尚不清楚。本研究利用16S rRNA基因高通量测序技术比较夏季野生和室内饲养高原鼠兔的肠道菌群发现,与野生鼠兔相比,室内鼠兔有更高丰度的拟杆菌门(Bacteroidetes)和螺旋体门(Spirochetes),更低丰度的变形菌门(Proteobacteria)和放线菌门(Actinobacteria)。野生和室内饲养的鼠兔肠道共有的操作分类单元(operational taxonomic units,OTUs)有50.1%是重叠的,而49.9%的OTUs是各自特有的。野生和室内饲养肠道菌群的组成和群落结构(β多样性)有显著性差异。因此,室内饲养鼠兔不能有效反映野生状态下高原鼠兔肠道菌群的情况。更多还原

【Abstract】 Gut microbiota play important roles in host physiological activity, immune regulation, and health. Although numerous studies have found that gut microbiota of plateau pikas are related to food and environment, the differences of gut microbiota between wild and laboratory-reared plateau pikas are still unclear. In this study, we used 16 S rRNA gene highthroughput sequencing technology to compare the differences of gut microbial communities between wild and laboratoryreared plateau pikas. Our results suggested that laboratory-reared plateau pikas had higher abundance of Bacteroidetes and Spirochetes, but lower abundance of Proteobacteria and Actinobacteria than wild plateau pikas. There was 50.1% overlap of gut operational taxonomic units(OTUs) between wild and laboratory-reared plateau pikas, while 49.9% of gut OTUs were specific in the wild and laboratory-reared plateau pikas. Significant differences were detected in both composition and community structure(β diversity) of gut microbes in the wild and laboratory-reared plateau pikas. Therefore, laboratoryreared plateau pika could not effectively reflect the gut microbiota of plateau pika in the wild.更多还原