【摘要】 【目的】针对毛白杨优树种质资源形态学差异小、不易区分等问题,利用多态SSR引物构建优树种质资源指纹图谱,为毛白杨种质资源管理、新品种选育、知识产权保护等提供参考。【方法】本研究以山东冠县毛白杨种质资源库469个优树无性系为对象,从毛白杨SSR多态引物筛选入手,通过荧光SSR引物PCR扩增和毛细管电泳仪检测筛选SSR引物,并构建指纹图谱。【结果】在2 317对SSR引物中,共计得到清晰、特异、多态、稳定扩增的SSR引物406对,这些SSR引物在19条染色体上的分布数量介于3～39对之间,利用BLAST比对分析可将其中389对SSR引物定位到毛果杨基因组,构建了毛白杨优树种质资源多态SSR引物库。应用多态性较高的SSR核心引物,以及特异的SSR辅助引物相结合的技术策略,筛选出25对多态性较高的SSR核心引物,以及13对特异的SSR辅助引物,构建了毛白杨种质资源库内469个优良无性系指纹图谱库,并完成了指纹图谱的QR编码。【结论】本研究成功构建了毛白杨种质资源多态SSR引物库和优树无性系指纹图谱库,对与毛白杨类似的林木种质资源遗传变异研究以及品种鉴定、系谱分析等具有重要意义。更多还原

【Abstract】 [Objective] Aimed at the problems that the morphological differences of superior tree germplasm resources of Populus tomentosa were small and difficult to distinguish, polymorphic SSR primers were used to construct the fingerprint of superior tree germplasm resources, provide references for the germplasm resource management, breeding of new cultivar, intellectual property protection, etc.[Method] In this study, 469 superior trees from Populus tomentosa germplasm resource bank in Guanxian County, Shandong Province of eastern China were used as material. Firstly, the SSR polymorphic primers were screened by PCR amplification and capillary electrophoresis detection, and then the fingerprint was constructed. [Result] In 2 317 pairs of SSR primers, a total of 406 pairs of SSR primers with clear, specific,polymorphic and stable amplification were obtained. These SSR primers distributed on 19 chromosomes and ranged in 3-39 pairs. Further, 389 pairs of SSR primers were located into the genome of P. trichocarpa based on BLAST comparison analysis, and a polymorphic SSR primer library of Populus tomentosa germplasm resources was constructed. On this basis, 25 pairs of SSR primers with high polymorphism and13 pairs of specific SSR-assisted primers were used to construct the fingerprint of 469 superior trees in the germplasm resource bank of Populus tomentosa, and the QR coding of the fingerprint was completed.[Conclusion] This study successfully constructed the polymorphic SSR primer library and the superior tree fingerprint library of Populus tomentosa germplasm resources, and be of great significance to the study of genetic variation, variety identification and pedigree analysis of Populus tomentosa.更多还原