【摘要】 【目的】盐害作为一类非生物胁迫严重危害了农作物的生存以及产量。Remorin作为一类植物特有的蛋白质在植物适应环境过程中具有重要功能。本研究克隆了胡杨remorin蛋白PeREM1. 3的编码基因PeREM1. 3,并研究PeREM1. 3基因在植物耐盐性中的作用。【方法】笔者将基因构件35S::PeREM1. 3转入模式植物烟草中,在盐胁迫条件下,通过生理生化的方法对表达PeREM1. 3的转基因烟草进行基因功能的分析。【结果】研究显示PeREM1. 3蛋白定位于细胞质膜上,其编码基因PeREM1. 3的开放阅读框(ORF)长600 bp,编码199个氨基酸。胡杨中PeREM1. 3能够响应盐胁迫和渗透胁迫表达上调。结果表明,在烟草中过表达PeREM1. 3明显地提高了耐盐性。过表达PeREM1. 3的烟草转基因株系中抗氧化物酶如SOD、POD和CAT活性显著提高,降低了活性氧水平,调控活性氧平衡。另外植物抗逆相关基因SOS1、HAK、NHA1、VAG1和PMA4的转录水平显著增高,调控K~+/Na~+平衡。【结论】这些结果说明PeREM1. 3蛋白通过维持植物的活性氧平衡和K~+/Na~+平衡来提高植物的耐盐性。更多还原

【Abstract】 [Objective]The tolerance to salt stress,which is a major abiotic stress,is critical to plant survival and productivity. Remorins are plant-specific proteins and play an important role in plant adaptation to adverse environments. In this study,the gene PeREM1. 3 coding for a remorin protein was cloned from Populus euphratica,and the role of PeREM1. 3 in plant salt tolerance was investigated.[Method]The gene constructs 35 S: : PeREM1. 3 was transferred to model plant tobacco to investigate the function of PeREM1. 3 in salt tolerance by physiological and biochemical methods. [Result]Studies showed that PeREM1. 3 protein localized on the plasma membrane. The 600 bp full-length of openreading frame( ORF) of PeREM1. 3 encoded a putative protein of 199 amino acids. The expression of PeREM1. 3 was up-regulated under salt and osmotic stress in Populus euphratica. The results showed that the expression of PeREM1. 3 increased the salt tolerance of tobacco. The activities of antioxidant enzymes such as SOD,POD and CAT significantly increased and decreased the ROS level to maintain ROS homeostasis in transgenic lines expressing PeREM1. 3 under salt stress. On the other hand, the transcriptional levels of plant stress-resistance related genes,such as SOS1,HAK,NHA1,VAG1 and PMA4,were significantly increased and then maintained the K~+/Na~+homeostasis in transgenic plants.[Conclusion]The above experimental data indicate that remorin protein could maintain ROS and K~+/Na~+homeostasis and then enhance plant salt tolerance.更多还原