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用mtDNA序列鉴定出上海海域须鲸科物种大村鲸
Identification of Balaenoptera omurai(Balaenopteridae) from Shanghai Seas by Using mtDNA Gene Sequences
【摘要】 测定了采自上海海域的一头死亡须鲸骨骼样本的线粒体DNA(mtDNA)细胞色素b基因(Cyt b)序列330 bp(登录号MK295815)、细胞色素C氧化酶Ⅰ基因(COⅠ)序列206 bp(登录号MK317953)和控制区(control region)序列231 bp(登录号MK317954)。通过GenBank中的BLAST分析,结果表明,样本的上述三部分序列与大村鲸(Balaenopteraomurai)对应序列的相似性均达到100%,基于最大似然法(ML)法构建的系统发育树与BLAST结果一致,故将标本鉴定为大村鲸,为上海海域首次记录。
【Abstract】 The portions of the mitochondrial DNA(mtDNA) Cyt b gene(330 bp, GenBank accession:MK295815), cytochrome c oxidase subunit I(COⅠ)(206 bp, GenBank accession: MK317953) and mitochondrial DNA(mtDNA) control region(231 bp, GenBank accession: MK317954) of the skeleton sample from a dead baleen whale collected at the Shanghai seas were sequenced. The blasting result in Gen Bank shows that the three sequences have maximal similarity at 100% with those of Balaenoptera omurai(Table 1﹣3); and the phylogenetic trees based on maximum likelihood are consistent with the blasting result(Fig. 5﹣7). The result shows that the sample should be identified as B. omurai, and it is recorded for the first time from Shanghai Seas.
- 【文献出处】 动物学杂志 ,Chinese Journal of Zoology , 编辑部邮箱 ,2019年04期
- 【分类号】Q959
- 【被引频次】2
- 【下载频次】75